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Human Integrin beta-7(ITGB7) ELISA kit

  • 中文名: 人整合素β-7(ITGB7)酶联免疫试剂盒
货号: IPD-EL011891HU
Price: ¥2500
数量:
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产品详情

  • 产品描述:
    人整合素β-7(ITGB7)酶联免疫试剂盒为双抗夹心法ELISA试剂盒,定量检测血清、血浆、组织匀浆样本中的ITGB7含量。ITGB7属于整合素家族成员,在免疫系统中发挥重要作用。它能介导细胞与细胞、细胞与细胞外基质间相互作用。研究中发现其与炎症性疾病、肿瘤转移等密切相关,通过干预ITGB7相关信号通路,有望为相关疾病治疗提供新思路。试剂盒检测范围为0.312 ng/mL-20 ng/mL,适用于科研领域中对肠道相关疾病模型、免疫细胞迁移机制或黏膜屏障功能的研究,例如通过检测炎症性肠病动物模型血清ITGB7水平变化,或分析肠道组织匀浆中该蛋白的表达动态,为探索免疫调控网络提供可靠数据支持。本品仅用于科研,不用于临床诊断,产品具体参数及操作步骤详见产品说明书。
  • 别名:
    ITGB7; Integrin beta-7; Gut homing receptor beta subunit
  • 缩写:
    ITGB7
  • Uniprot No.:
    P26010
  • 种属:
    Homo sapiens (Human)
  • 样本类型:
    serum, plasma, tissue homogenates
  • 检测范围:
    0.312 ng/mL-20 ng/mL
  • 灵敏度:
    0.078 ng/mL
  • 反应时间:
    1-5h
  • 样本体积:
    50-100ul
  • 检测波长:
    450 nm
  • 研究领域:
    Signal Transduction
  • 测定原理:
    quantitative
  • 测定方法:
    Sandwich
  • 精密度:

    Intra-assay Precision (Precision within an assay): CV%<8%




    Three samples of known concentration were tested twenty times on one plate to assess.


    Inter-assay Precision (Precision between assays): CV%<10%




    Three samples of known concentration were tested in twenty assays to assess.



  • 线性度:

    To assess the linearity of the assay, samples were spiked with high concentrations of human ITGB7 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.


    Sample

    Serum(n=4)


    1:1

    Average %

    102


    Range %

    95-108


    1:2

    Average %

    97


    Range %

    92-103


    1:4

    Average %

    91


    Range %

    87-90


    1:8

    Average %

    93


    Range %

    88-99


  • 回收率:

    The recovery of human ITGB7 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

    Sample Type

    Average % Recovery

    Range


    Serum (n=5)

    93

    86-100


    EDTA plasma (n=4)

    96

    90-101


  • 标准曲线:

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.







    ng/ml

    OD1

    OD2

    Average

    Corrected


    20

    3.028

    2.934

    2.981

    2.843


    10

    2.523

    2.667

    2.595

    2.457


    5

    1.888

    1.928

    1.908

    1.771


    2.5

    1.182

    1.229

    1.206

    1.068


    1.25

    0.685

    0.721

    0.703

    0.566


    0.625

    0.421

    0.433

    0.427

    0.290


    0.312

    0.276

    0.284

    0.280

    0.142


    0

    0.140

    0.135

    0.138




  • 货期:
    3-5 working days

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