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产品描述：人间α-胰蛋白酶抑制因子重链H1(ITIH1)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、尿液、组织匀浆样本中的ITIH1含量。试剂盒检测范围为15.6 ng/mL-1000 ng/mL，本试剂盒可应用于基础医学研究中ITIH1的生物学功能解析、炎症相关疾病模型的分子机制探索，以及药物干预对细胞外基质重塑影响的体外评价等科研场景。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：H1P ELISA Kit; IATIH ELISA Kit; IGHEP1 ELISA Kit; Inter alpha (globulin) inhibitor H1 ELISA Kit; Inter alpha (globulin) inhibitor H1 polypeptide ELISA Kit; Inter alpha trypsin inhibitor heavy chain 1 ELISA Kit; Inter alpha trypsin inhibitor heavy chain H1 ELISA Kit; Inter-alpha-inhibitor heavy chain 1 ELISA Kit; Inter-alpha-trypsin inhibitor complex component III ELISA Kit; Inter-alpha-trypsin inhibitor heavy chain H1 ELISA Kit; ITI heavy chain H1 ELISA Kit; ITI-HC1 ELISA Kit; ITIH ELISA Kit; ITIH1 ELISA Kit; ITIH1_HUMAN ELISA Kit; Serum-derived hyaluronan-associated protein ELISA Kit; SHAP ELISA Kit
缩写：ITIH1
Uniprot No.：P19827
种属：Homo sapiens (Human)
样本类型：serum, plasma, cell culture supernates, urine, tissue homogenates
检测范围：15.6 ng/mL-1000 ng/mL
灵敏度：3.9 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cell Biology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human ITIH1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:100 | Average % | 86 | ||||
| Range % | 80-92 | |||||
| 1:200 | Average % | 97 | ||||
| Range % | 91-105 | |||||
| 1:400 | Average % | 99 | ||||
| Range % | 92-110 | |||||
| 1:800 | Average % | 97 | ||||
| Range % | 86-98 | |||||
回收率：
| The recovery of human ITIH1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 93 | 89-98 | ||||
| EDTA plasma (n=4) | 97 | 90-104 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected10002.4002.4182.4092.3285002.0881.9992.0041.9232501.5621.5551.5591.4781251.2281.2111.2201.13962.50.6890.6650.6770.59631.250.4520.4360.4440.36315.60.2700.2540.2620.18100.0840.0780.081 | ||||||
货期：3-5 working days
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