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产品描述：人间-alpha-胰蛋白酶抑制剂重链H4(ITIH4/IHRP/ITIHL1/PK120/PRO1851)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的ITIH4含量。当前所给信息未包含 ITIH4 相关背景及研究机制内容，无法总结。若你能提供更多有关 ITIH4 背景、研究机制的详细文本，我会为你精炼总结成 100 字左右的段落。试剂盒检测范围为0.625 ng/mL-40 ng/mL，支持科研人员探究ITIH4在疾病发生发展中的作用机制、药物干预效果评估或炎症相关通路研究，为分子生物学、病理学及药理学领域的基础研究提供可靠工具，适用于体外定量分析多种生物样本中ITIH4的表达水平。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：35 kDa inter-alpha-trypsin inhibitor heavy chain H4 ELISA Kit; gp120 ELISA Kit; H4P ELISA Kit; IHRP ELISA Kit; Inter-alpha-inhibitor heavy chain 4 ELISA Kit; Inter-alpha-trypsin inhibitor family heavy chain-related protein ELISA Kit; Inter-alpha-trypsin inhibitor heavy chain H4 ELISA Kit; inter-alpha-trypsin inhibitor, heavy chain-like, 1 ELISA Kit; ITI heavy chain H4 ELISA Kit; ITI-HC4 ELISA Kit; ITIH4 ELISA Kit; ITIH4_HUMAN ELISA Kit; ITIHL1 ELISA Kit; OTTHUMP00000197120 ELISA Kit; OTTHUMP00000197121 ELISA Kit; OTTHUMP00000213834 ELISA Kit; OTTHUMP00000213869 ELISA Kit; PK-120 ELISA Kit; PK120 ELISA Kit; Plasma kallikrein sensitive glycoprotein 120 ELISA Kit; PRO1851 ELISA Kit
缩写：ITIH4
Uniprot No.：Q14624
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：0.625 ng/mL-40 ng/mL
灵敏度：0.156 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human ITIH4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 87 | ||||
| Range % | 82-97 | |||||
| 1:2 | Average % | 97 | ||||
| Range % | 92-102 | |||||
| 1:4 | Average % | 96 | ||||
| Range % | 90-104 | |||||
| 1:8 | Average % | 97 | ||||
| Range % | 92-102 | |||||
回收率：
| The recovery of human ITIH4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 106 | 98-110 | ||||
| EDTA plasma (n=4) | 95 | 91-105 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected 402.5392.4382.4892.329 202.0471.9461.9971.837 101.3121.3011.3071.147 50.7550.7660.7610.601 2.50.4630.4520.4580.298 1.250.3370.3260.3320.172 0.6250.2420.2450.2440.084 00.1600.1590.160 | ||||||
货期：3-5 working days
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