| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | STMN4 |
| Uniprot No | Q9H169 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-216aa |
| 氨基酸序列 | MTLAAYKEKMKELPLVSLFCSCFLADPLNKSSYKYEGWCGRQCRRKDESQRKDSADWRERRAQADTVDLNWCVISDMEVIELNKCTSGQSFEVILKPPSFDGVPEFNASLPRRRDPSLEEIQKKLEAAEERRKYQEAELLKHLAEKREHEREVIQKAIEENNNFIKMAKEKLAQKMESNKENREAHLAAMLERLQEKDKHAEEVRKNKELKEEASR |
| 预测分子量 | 52.4kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于STMN4重组蛋白的3篇参考文献及其摘要概括：
1. **文献名称**: "Recombinant human stathmin-4 (STMN4) promotes axonal regeneration in vitro via microtubule dynamics modulation"
**作者**: Li, X. et al.
**摘要**: 研究报道了人源重组STMN4蛋白的制备及功能，发现其通过调节微管解聚促进神经元轴突再生，提示其在神经修复中的潜在应用。
2. **文献名称**: "Expression and purification of biologically active STMN4 in E. coli for functional studies"
**作者**: Park, J.H. & Kim, S.
**摘要**: 描述了利用大肠杆菌系统高效表达可溶性STMN4重组蛋白的优化方法，并验证其通过体外微管结合实验保留生物学活性。
3. **文献名称**: "STMN4 knockdown inhibits glioma cell invasion by destabilizing microtubules: Role of recombinant STMN4 in rescue experiments"
**作者**: Chen, R. et al.
**摘要**: 研究利用重组STMN4蛋白恢复胶质瘤细胞中STMN4缺失的表型，证明其通过稳定微管结构促进肿瘤细胞侵袭，为癌症治疗提供靶点线索。
注：上述文献为模拟示例，实际研究中建议通过PubMed或Web of Science以“STMN4 recombinant”或“Stathmin-4 expression”为关键词检索最新成果。若需具体文献，可提供更详细的研究方向进一步筛选。
**Background of STMN4 Recombinant Protein**
STMN4 (Stathmin-4), also known as RB3. is a member of the stathmin family of phosphoproteins, which regulate microtubule dynamics by interacting with tubulin. Unlike its well-studied homolog STMN1 (Stathmin-1), STMN4 exhibits tissue-specific expression, predominantly in the nervous system, where it plays roles in neuronal development, plasticity, and axonal regeneration. It modulates microtubule stability by sequestering free tubulin or promoting depolymerization, thereby influencing cell shape, intracellular transport, and signaling.
The recombinant STMN4 protein is produced via genetic engineering, often using bacterial or mammalian expression systems, to ensure high purity and activity for research applications. Its recombinant form enables precise study of STMN4's structure-function relationships, post-translational modifications (e.g., phosphorylation), and interactions with binding partners.
Research on STMN4 has implications for neurodegenerative diseases (e.g., Alzheimer’s, Parkinson’s) and nerve injury recovery, as microtubule dysregulation is linked to these conditions. Additionally, STMN4’s restricted neural expression makes it a potential biomarker or therapeutic target for neurological disorders. Studies also explore its role in cancer, though its involvement remains less defined compared to STMN1.
Overall, STMN4 recombinant protein serves as a critical tool for deciphering microtubule-related mechanisms in health and disease, bridging gaps in understanding neural-specific cytoskeletal regulation.
×
