首页 / 产品 / ELISA试剂盒 / 大鼠ELISA试剂盒
产品描述：大鼠KI-67酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的KI-67含量。KI-67 是一种与细胞增殖相关的核抗原。其表达水平与细胞增殖活性密切相关，在正常组织和肿瘤组织中有不同表现。研究中常将其作为评估细胞增殖状态的重要指标，在肿瘤诊断、预后判断等方面有重要价值，用于指导治疗方案的制定。试剂盒检测范围为0.156 ng/mL-10 ng/mL，肿瘤生长评估、组织再生研究、体外培养细胞增殖分析、肿瘤模型研究、药物作用机制探索、基础研究中对细胞动态增殖行为的定量化分析本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Nifk ELISA Kit; Mki67ipMKI67 FHA domain-interacting nucleolar phosphoprotein ELISA Kit; Nucleolar protein interacting with the FHA domain of pKI-67 ELISA Kit
缩写：KI-67
Uniprot No.：Q5RJM0
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：0.156 ng/mL-10 ng/mL
灵敏度：0.04 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cancer
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat KI-67 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 87 | ||||
| Range % | 83-90 | |||||
| 1:2 | Average % | 93 | ||||
| Range % | 90-96 | |||||
| 1:4 | Average % | 89 | ||||
| Range % | 85-93 | |||||
| 1:8 | Average % | 100 | ||||
| Range % | 95-105 | |||||
回收率：
| The recovery of rat KI-67 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 89 | 85-93 | ||||
| EDTA plasma (n=4) | 92 | 88-96 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected102.7962.7312.7642.56652.4472.4072.4272.2292.51.7141.7681.7411.5431.251.0291.0681.0490.8510.6250.6140.6380.6260.4280.3120.4730.4980.4860.2880.1560.3310.3430.3370.13900.1970.1990.198 | ||||||
货期：3-5 working days
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