Canine kidney injury molecule 1(Kim-1) ELISA Kit

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

货号: IPD-E13035c

Price： ￥3000

48T 96T

数量：

大包装询价

产品描述：
犬肾损伤分子1(Kim-1)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的Kim-1含量。Kim-1即肾损伤分子-1，是肾小管上皮细胞在肾损伤时表达上调的标志物。在肾脏疾病中，其背景与早期肾损伤密切相关。研究机制上，Kim-1可促进巨噬细胞极化、调节炎症反应，还参与肾小管上皮细胞修复与再生过程，是肾损伤研究的重要靶点。试剂盒检测范围为0.312 ng/mL-20 ng/mL，科研人员可将其用于犬类肾脏疾病的机制研究，例如急性肾损伤模型的生物标志物动态监测、药物或化学物质肾毒性评估，以及慢性肾病进展的相关性分析。兼容组织匀浆样本的特性支持离体肾脏组织的蛋白表达研究，而血清和血浆的检测能力则为体内样本的实时分析提供便利，尤其适用于纵向研究中多次采样数据的对比。是探索犬类肾脏病理生理机制及治疗干预效果的重要工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：
Kim-1
种属：
Canis lupus familiaris (Dog) (Canis familiaris)
样本类型：
serum, plasma, tissue homogenates
检测范围：
0.312 ng/mL-20 ng/mL
灵敏度：
0.078 ng/ml.
反应时间：
1-5h
样本体积：
50-100ul
检测波长：
450 nm
研究领域：
Immunology
测定原理：
quantitative
测定方法：
Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of canine Kim-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

	Sample	Serum(n=4)
1:1	Average %	91
	Range %	86-95
1:2	Average %	102
	Range %	97-107
1:4	Average %	91
	Range %	85-97
1:8	Average %	97
	Range %	91-103

回收率：

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.


ng/ml	OD1	OD2	Average	Corrected
20	2.449	2.563	2.506	2.348
10	1.595	1.538	1.567	1.409
5	1.001	1.052	1.027	0.869
2.5	0.687	0.648	0.668	0.510
1.25	0.463	0.473	0.468	0.310
0.625	0.306	0.314	0.310	0.152
0.312	0.221	0.232	0.227	0.069
0	0.154	0.161	0.158