首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠干细胞因子/肥大细胞生长因子(SCF/MGF)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的KITLG含量。KITLG（KIT配体）是一种由骨髓微环境中的基质细胞产生的酸性糖蛋白，通过与其受体c-Kit结合，激活下游信号传导途径，如JAK/STAT、PI3K/AKT、ERK1/2和STAT3等，从而促进细胞增殖、分化和迁移。KITLG的异常表达与多种疾病相关，包括家族性进行性高色素沉着症、神经性耳聋、某些类型的癌症等，是癌症治疗中的潜在药物靶点。试剂盒检测范围为3.12 pg/mL-200 pg/mL，适用于干细胞生物学、免疫调控机制、组织再生等基础研究领域，通过检测血液或组织样本中SCF/MGF的表达水平，可深入探究其在炎症反应、肿瘤微环境或再生医学中的调控作用，为科研人员提供稳定高效的定量分析工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Kitlg; Kitl; Mgf; Sl; Slf; Kit ligand; Hematopoietic growth factor KL; Mast cell growth factor; MGF; Steel factor; Stem cell factor; SCF; c-Kit ligand
缩写：KITLG
Uniprot No.：P20826
种属：Mus musculus (Mouse)
样本类型：serum, plasma, tissue homogenates
检测范围：3.12 pg/mL-200 pg/mL
灵敏度：0.78 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse SCF/MGF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 83 | |||||
| Range % | 80-85 | ||||||
| 1:2 | Average % | 100 | |||||
| Range % | 97-103 | ||||||
| 1:4 | Average % | 86 | |||||
| Range % | 83-89 | ||||||
| 1:8 | Average % | 108 | |||||
| Range % | 104-112 | ||||||
回收率：
| The recovery of mouse SCF/MGF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 99 | 95-103 | |||||
| EDTA plasma (n=4) | 86 | 83-89 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| pg/mlOD1OD2AverageCorrected2001.8041.9001.8521.7351001.1951.2011.1981.081500.7310.7490.7400.623250.4480.4380.4430.32612.50.3090.3060.3080.1916.250.2050.2100.2080.0913.120.1510.1500.1510.03400.1180.1160.117 | |||||||
货期：3-5 working days
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