首页 / 产品 / ELISA试剂盒 / 大鼠ELISA试剂盒
产品描述：大鼠干细胞因子/肥大细胞生长因子(SCF/MGF)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的KITLG含量。KITLG是一种重要靶点。它在细胞增殖、分化和存活等过程有作用，参与造血干细胞维持、黑素细胞发育等生理活动。研究聚焦于其信号通路及与疾病关联，探索以其为靶点开发治疗癌症、血液疾病等药物的机制。试剂盒检测范围为6.25 pg/mL-400 pg/mL，支持科研领域探究SCF/MGF在血液系统疾病模型、组织损伤修复机制或免疫微环境调控中的表达变化，例如骨髓微环境研究、皮肤/肠道组织再生实验，或过敏性疾病模型中肥大细胞活化相关机制探索。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Kitlg; Kitl; Mgf; Kit ligand; Hematopoietic growth factor KL; Mast cell growth factor; MGF; Stem cell factor; SCF; c-Kit ligand
缩写：KITLG
Uniprot No.：P21581
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma, tissue homogenates
检测范围：6.25 pg/mL-400 pg/mL
灵敏度：1.56 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat SCF/MGF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 100 | ||||
| Range % | 95-106 | |||||
| 1:2 | Average % | 97 | ||||
| Range % | 91-105 | |||||
| 1:4 | Average % | 88 | ||||
| Range % | 84-92 | |||||
| 1:8 | Average % | 93 | ||||
| Range % | 86-98 | |||||
回收率：
| The recovery of rat SCF/MGF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 95 | 89-98 | ||||
| EDTA plasma (n=4) | 92 | 88-96 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected4002.8152.7522.7842.6002002.1342.0762.1051.9211001.3681.3191.3441.160500.9200.9340.9270.743250.5460.5270.5370.35312.50.4030.4210.4120.2286.250.2630.2480.2560.07200.1830.1850.184 | ||||||
货期：3-5 working days
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