Rat Klotho ELISA Kit

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

货号: IPD-E14958r

Price： ￥2500

48T 96T

数量：

大包装询价

产品描述：
大鼠 Klotho酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的KL含量。试剂盒检测范围为125 pg/mL-8000 pg/mL，适用于基础科研中探索Klotho在衰老机制、肾脏疾病模型、代谢综合征或药物干预研究中的表达动态，例如通过检测不同生理状态或实验处理后动物血清、组织中的Klotho浓度变化，为相关分子机制研究及潜在生物标志物筛选提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：
KlKlotho ELISA Kit; EC 3.2.1.31) [Cleaved into: Klotho peptide] ELISA Kit
缩写：
KL
Uniprot No.：
Q9Z2Y9
种属：
Rattus norvegicus (Rat)
样本类型：
serum, plasma, tissue homogenates
检测范围：
125 pg/mL-8000 pg/mL
灵敏度：
31.25 pg/mL
反应时间：
1-5h
样本体积：
50-100ul
检测波长：
450 nm
研究领域：
Developmental Biology
测定原理：
quantitative
测定方法：
Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat klotho in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

	Sample	Serum(n=4)
1:1	Average %	108
	Range %	103-114
1:2	Average %	92
	Range %	88-97
1:4	Average %	103
	Range %	98-107
1:8	Average %	93
	Range %	88-98

回收率：

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.


pg/ml	OD1	OD2	Average	Corrected
8000	2.678	2.634	2.656	2.495
4000	2.231	2.107	2.169	2.008
2000	1.602	1.578	1.590	1.429
1000	1.006	1.029	1.018	0.857
500	0.658	0.628	0.643	0.482
250	0.429	0.433	0.431	0.270
125	0.308	0.294	0.301	0.140
0	0.157	0.165	0.161