首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠Klotho酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液样本中的KL含量。Klotho基因是1997年被发现的与人类衰老密切相关的基因，其缺陷表达会导致类似人类衰老的表型，而过表达则能延长小鼠寿命。Klotho通过抑制Insulin/IGF-1信号通路发挥其生物学功能，包括抑制衰老、抵抗氧化应激和影响肿瘤发生发展。试剂盒检测范围为3.12 pg/mL-200 pg/mL，适用于体外培养细胞模型中Klotho分泌水平的动态监测，或实验小鼠模型（如慢性肾病造模、衰老干预研究）中血清/血浆生物标志物的定量分析。科研人员可通过探究Klotho在衰老机制、代谢调控网络中的作用，或用于评估药物干预、基因编辑等实验手段对Klotho表达的影响。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Kl ELISA Kit; Klotho ELISA Kit; EC 3.2.1.31) [Cleaved into: Klotho peptide] ELISA Kit
缩写：KL
Uniprot No.：O35082
种属：Mus musculus (Mouse)
样本类型：serum, plasma, cell culture supernates
检测范围：3.12 pg/mL-200 pg/mL
灵敏度：0.78 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Developmental Biology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse klotho in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 84 | ||||
| Range % | 80-90 | |||||
| 1:2 | Average % | 96 | ||||
| Range % | 90-101 | |||||
| 1:4 | Average % | 92 | ||||
| Range % | 87-99 | |||||
| 1:8 | Average % | 95 | ||||
| Range % | 90-102 | |||||
回收率：
| The recovery of mouse klotho spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 97 | 91-104 | ||||
| EDTA plasma (n=4) | 98 | 92-102 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected2002.1572.1072.1321.9681001.3721.3341.3531.189500.8050.7980.8020.638250.5160.5040.5100.34612.50.3480.3310.3400.1766.250.2730.2580.2660.1023.120.2280.2150.2220.05800.1680.1600.164 | ||||||
货期：3-5 working days
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