首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人β-克洛素(KLB)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的KLB含量。KLB（Klotho beta）是一种蛋白质编码基因，参与RET信号通路和HIV生命周期。其在多种代谢过程中起调节作用，但在癌症中的作用尚不明确。研究发现，βKlotho在肝癌组织中的表达下调，且其重新引入肝癌细胞可抑制其增殖。KLB的研究机制涉及FGF19/21-KLB-FGFRs信号通路，该通路在脂质代谢和抗肥胖中发挥作用。试剂盒检测范围为62.5 pg/mL-4000 pg/mL，适用于体外实验中对KLB蛋白表达量的动态监测，如探索其在肥胖、糖尿病、非酒精性脂肪肝等代谢相关疾病中的分子机制，或用于药物筛选模型中评估化合物对KLB表达的影响。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Beta-klotho ELISA Kit; BetaKlotho ELISA Kit; BKL ELISA Kit; KLB ELISA Kit; KLOTB_HUMAN ELISA Kit; Klotho beta like protein ELISA Kit; Klotho beta-like protein ELISA Kit
缩写：KLB
Uniprot No.：Q86Z14
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：62.5 pg/mL-4000 pg/mL
灵敏度：15.6 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Metabolism
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human KLB in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 102 | ||||
| Range % | 96-105 | |||||
| 1:2 | Average % | 88 | ||||
| Range % | 84-92 | |||||
| 1:4 | Average % | 94 | ||||
| Range % | 89-98 | |||||
| 1:8 | Average % | 92 | ||||
| Range % | 88-94 | |||||
回收率：
| The recovery of human KLB spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 102 | 96-106 | ||||
| EDTA plasma (n=4) | 88 | 82-92 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected40002.3722.2782.3252.22320001.7461.8441.7951.69310001.3961.2931.3451.2435000.9720.9610.9670.8652500.5880.5760.5820.4801250.3540.3680.3610.25962.50.2250.2300.2280.12600.1030.1010.102 | ||||||
货期：3-5 working days
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