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产品描述:人激肽释放酶6(KLK 6)酶联免疫试剂盒为双抗夹心法ELISA试剂盒,定量检测血清、血浆、组织匀浆样本中的KLK6含量。KLK6 是一种重要的靶点。它属于激肽释放酶家族成员,在神经系统、免疫系统等多种生理过程中发挥作用。目前研究聚焦于其在肿瘤、神经退行性疾病等发病机制里的角色,探索以它为靶点开发诊断标志物或治疗药物。试剂盒检测范围为1.25 ng/mL-80 ng/mL,适用于基础科研中探索KLK6在疾病模型中的表达规律、评估其作为生物标志物的潜力,或用于药物干预实验中的动态监测。兼容多种生物样本类型,包括血清、血浆及组织匀浆,满足体外实验中对KLK6蛋白水平的定量分析需求,为神经科学、肿瘤学及分子机制研究提供可靠工具。本品仅用于科研,不用于临床诊断,产品具体参数及操作步骤详见产品说明书。
别名:Bssp ELISA Kit; hK 6 ELISA Kit; hK6 ELISA Kit; Kallikrein 6 precursor ELISA Kit; Kallikrein related peptidase 6 ELISA Kit; Kallikrein-6 ELISA Kit; Kallikrein6 ELISA Kit; KLK 6 ELISA Kit; Klk 7 ELISA Kit; KLK 9 ELISA Kit; Klk29 ELISA Kit; Klk6 ELISA Kit; KLK6_HUMAN ELISA Kit; Klk7 ELISA Kit; KLK9 ELISA Kit; MGC9355 ELISA Kit; mGK 1 ELISA Kit; mGK1 ELISA Kit; MSP ELISA Kit; Neurosin ELISA Kit; Protease M ELISA Kit; Protease serine 18 ELISA Kit; Protease serine 9 ELISA Kit; PRSS 18 ELISA Kit; PRSS 9 ELISA Kit; PRSS18 ELISA Kit; PRSS9 ELISA Kit; Serine protease 18 ELISA Kit; Serine protease 9 ELISA Kit; SP 59 ELISA Kit; SP59 ELISA Kit; Tissue kallikrein ELISA Kit; TK ELISA Kit; Zyme ELISA Kit
缩写:KLK6
Uniprot No.:Q92876
种属:Homo sapiens (Human)
样本类型:serum, plasma, tissue homogenates
检测范围:1.25 ng/mL-80 ng/mL
灵敏度:0.31 ng/mL
反应时间:1-5h
样本体积:50-100ul
检测波长:450 nm
研究领域:Cancer
测定原理:quantitative
测定方法:Sandwich
精密度:
Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
Inter-assay Precision (Precision between assays): CV%<10% | ||||||
Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度:
To assess the linearity of the assay, samples were spiked with high concentrations of human KLK 6 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
Sample | Serum(n=4) | |||||
1:1 | Average % | 89 | ||||
Range % | 83-95 | |||||
1:2 | Average % | 95 | ||||
Range % | 91-99 | |||||
1:4 | Average % | 95 | ||||
Range % | 88-102 | |||||
1:8 | Average % | 97 | ||||
Range % | 91-101 |
回收率:
The recovery of human KLK 6 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
Sample Type | Average % Recovery | Range | ||||
Serum (n=5) | 86 | 80-93 | ||||
EDTA plasma (n=4) | 96 | 92-103 | ||||
标准曲线:
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
ng/mlOD1OD2AverageCorrected 802.8192.9602.8902.737 402.2372.2052.2212.068 201.5051.5861.5461.393 100.7990.7550.7770.624 50.4420.4110.4270.274 2.50.3130.3220.3180.165 1.250.2450.2350.2400.087 00.1540.1520.153 |
货期:3-5 working days
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