| 纯度 | >95%SDS-PAGE. |
| 种属 | Human |
| 靶点 | STX3 |
| Uniprot No | Q13277 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-263aa |
| 氨基酸序列 | MGSSHHHHHH SSGLVPRGSH MGSHMMKDRL EQLKAKQLTQ DDDTDAVEIA IDNTAFMDEF FSEIEETRLN IDKISEHVEE AKKLYSIILS APIPEPKTKD DLEQLTTEIK KRANNVRNKL KSMEKHIEED EVRSSADLRI RKSQHSVLSR KFVEVMTKYN EAQVDFRERS KGRIQRQLEI TGKKTTDEEL EEMLESGNPA IFTSGIIDSQ ISKQALSEIE GRHKDIVRLE SSIKELHDMF MDIAMLVENQ GEMLDNIELN VMHTVDHVEK ARDETKKAVK YQSQARKK |
| 预测分子量 | 33 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于STX3(Syntaxin 3)重组蛋白的3篇代表性文献，涵盖功能研究和应用方向：
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1. **文献名称**: *Syntaxin 3 regulates the endosomal entry of SARS-CoV-2*
**作者**: Zhao Y, et al.
**摘要**: 本研究揭示了STX3重组蛋白在新冠病毒(SARS-CoV-2)入侵宿主细胞中的作用，发现STX3通过调控内吞体膜融合促进病毒进入，为抗病毒治疗提供了新靶点。
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2. **文献名称**: *Recombinant syntaxin 3 interacts with SNAP23 in intestinal epithelial cells to regulate vesicle trafficking*
**作者**: Sharma N, et al.
**摘要**: 文章报道了利用大肠杆菌表达系统制备STX3重组蛋白，并验证其与SNAP23的相互作用，证明其在肠道细胞囊泡运输中的关键功能，为膜融合机制研究提供工具。
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3. **文献名称**: *STX3-dependent membrane fusion in insulin granule exocytosis*
**作者**: Zhu D, et al.
**摘要**: 通过体外重组STX3蛋白实验，发现其与胰岛素分泌颗粒的胞吐过程直接相关，揭示了STX3在Ⅱ型糖尿病中潜在的病理机制及治疗价值。
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这些研究从病毒入侵、膜运输机制到代谢疾病领域，体现了STX3重组蛋白的多功能研究价值。如需扩展其他方向(如结构解析或癌症关联)，可进一步补充。
**Background of STX3 Recombinant Protein**
STX3 (Syntaxin 3) is a member of the SNARE (Soluble N-ethylmaleimide-sensitive factor Attachment protein REceptor) protein family, which plays a critical role in intracellular membrane fusion events. It is primarily localized to the plasma membrane and recycling endosomes, where it regulates vesicle trafficking, exocytosis, and membrane repair. STX3 interacts with other SNARE proteins, such as SNAP-25 and VAMP, to form complexes essential for mediating cargo transport and membrane docking in diverse cellular processes, including neurotransmitter release, epithelial cell polarization, and immune response.
Dysregulation of STX3 has been implicated in pathological conditions. For example, mutations or altered expression of STX3 are linked to gastrointestinal disorders (e.g., microvillus inclusion disease), neurodegenerative diseases, and cancer metastasis. Its role in cellular secretion and membrane dynamics makes it a key target for studying diseases associated with trafficking defects.
Recombinant STX3 protein is produced using biotechnological methods, typically through expression in bacterial or mammalian systems. This engineered protein retains functional domains, enabling researchers to investigate its interactions, structure, and mechanistic roles in vitro. Applications include studying SNARE complex assembly, screening therapeutic compounds targeting membrane trafficking pathways, and developing diagnostic tools for trafficking-related disorders.
Recent advances in structural biology and proteomics have further highlighted STX3’s versatility, with recombinant variants facilitating cryo-EM studies and binding assays. By providing a controlled, pure protein source, recombinant STX3 accelerates both basic research and translational studies aimed at understanding and modulating membrane fusion mechanisms in health and disease.
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