首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠血浆激肽释放酶(KLKB1)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的KLKB1含量。试剂盒检测范围为3.12 ng/mL-200 ng/mL，支持基础科研中KLKB1相关机制研究，例如炎症模型中的激肽系统动态分析、药物干预对凝血 - 纤溶平衡的影响评估，或代谢性疾病动物模型中酶活性变化的探索。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Klkb1 ELISA Kit; Klk3 ELISA Kit; PkPlasma kallikrein ELISA Kit; EC 3.4.21.34 ELISA Kit; Fletcher factor ELISA Kit; Kininogenin ELISA Kit; Plasma prekallikrein) [Cleaved into: Plasma kallikrein heavy chain; Plasma kallikrein light chain] ELISA Kit
缩写：KLKB1
Uniprot No.：P26262
种属：Mus musculus (Mouse)
样本类型：serum, plasma, tissue homogenates
检测范围：3.12 ng/mL-200 ng/mL
灵敏度：0.78 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cell Biology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse KLKB1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1000 | Average % | 99 | ||||
| Range % | 96-102 | |||||
| 1:2000 | Average % | 103 | ||||
| Range % | 100-106 | |||||
| 1:4000 | Average % | 89 | ||||
| Range % | 84-94 | |||||
| 1:8000 | Average % | 96 | ||||
| Range % | 94-98 | |||||
回收率：
| The recovery of mouse KLKB1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 93 | 89-97 | ||||
| EDTA plasma (n=4) | 103 | 100-105 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected 2002.4592.5552.5072.388 1001.8771.9661.9221.803 501.3551.3321.3441.225 250.7890.7680.7790.660 12.50.5460.5880.5670.448 6.250.3230.3450.3340.215 3.120.2040.2210.2130.094 00.1180.1190.119 | ||||||
货期：3-5 working days
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