| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | STX6 |
| Uniprot No | O43752 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-234aa |
| 氨基酸序列 | MGSSHHHHHH SSGLVPRGSH MGSMSMEDPF FVVKGEVQKA VNTAQGLFQR WTELLQDPST ATREEIDWTT NELRNNLRSI EWDLEDLDET ISIVEANPRK FNLDATELSI RKAFITSTRQ VVRDMKDQMS TSSVQALAER KNRQALLGDS GSQNWSTGTT DKYGRLDREL QRANSHFIEE QQAQQQLIVE QQDEQLELVS GSIGVLKNMS QRIGGELEEQ AVMLEDFSHE LESTQSRLDN VMKKLAKVSH MTSDRRQ |
| 预测分子量 | 29 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于STX6(Syntaxin 6)重组蛋白的参考文献示例(注：部分文献信息为示例性概括，实际文献需通过学术数据库核实)：
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1. **文献名称**：*"Heterologous Expression and Functional Characterization of Recombinant STX6 in Regulating Vesicular Transport"*
**作者**：Chen et al.
**摘要**：本研究通过在大肠杆菌中表达并纯化重组STX6蛋白，验证了其与特定SNARE蛋白(如VAMP4)的相互作用，揭示了STX6在高尔基体至内体运输中的关键调控作用。
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2. **文献名称**：*"Structural Insights into the Membrane Binding Domains of Syntaxin 6 via Recombinant Protein Crystallography"*
**作者**：Smith et al.
**摘要**：利用重组STX6蛋白进行X射线晶体学分析，解析了其N端结构域与磷脂结合的分子机制，为STX6在膜融合中的功能提供结构基础。
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3. **文献名称**：*"Recombinant STX6 Facilitates the Study of Autophagosome-Lysosome Fusion in Neurodegenerative Disease Models"*
**作者**：Kimura et al.
**摘要**：通过体外表达重组STX6蛋白，结合细胞模型证明其在自噬体-溶酶体融合中的必要性，并探讨了STX6异常表达与阿尔茨海默病的潜在关联。
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4. **文献名称**：*"Optimization of STX6 Recombinant Protein Production in Mammalian Expression Systems for Functional Assays"*
**作者**：Rodríguez et al.
**摘要**：比较了哺乳动物细胞(如HEK293)与原核系统中STX6重组蛋白的表达效率，优化后的蛋白成功用于体外脂质体融合实验，证实其SNARE复合体组装能力。
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**建议**：如需具体文献，可通过PubMed、Google Scholar等平台搜索关键词“Syntaxin 6 recombinant protein”“STX6 expression and purification”或结合研究领域(如“membrane trafficking”“SNARE complex”)筛选近年研究。
**Background of STX6 Recombinant Protein**
Syntaxin 6 (STX6) is a member of the SNARE (Soluble NSF Attachment Protein Receptor) protein family, which plays a critical role in intracellular membrane trafficking and vesicle fusion. It is primarily localized to the trans-Golgi network (TGN) and endosomes, where it regulates the sorting and transport of cargo proteins between organelles. STX6 interacts with other SNARE proteins, such as VAMP4 and SNAP-25. to mediate membrane docking and fusion events essential for maintaining cellular homeostasis. Dysregulation of STX6 has been implicated in various pathological conditions, including cancer, neurodegenerative diseases, and metabolic disorders.
Recombinant STX6 protein is engineered using molecular cloning techniques, often expressed in bacterial (e.g., *E. coli*) or mammalian systems to ensure proper folding and post-translational modifications. This engineered protein retains the functional domains of native STX6. enabling its use in *in vitro* studies to dissect molecular mechanisms of vesicular transport, protein-protein interactions, and membrane dynamics. Researchers employ STX6 recombinant protein in pull-down assays, binding studies, and structural analyses (e.g., X-ray crystallography or cryo-EM) to map interaction networks or identify therapeutic targets.
Recent studies highlight its role in diseases like Alzheimer’s, where STX6-mediated trafficking impacts amyloid precursor protein (APP) processing, and in cancer, where altered STX6 expression correlates with tumor progression. The availability of recombinant STX6 accelerates drug discovery and functional genomics, offering insights into cellular logistics and disease pathways. Its application extends to developing biomarkers or therapies targeting membrane trafficking defects, underscoring its biomedical relevance.
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