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产品描述：小鼠淋巴细胞活化基因3蛋白(LAG3)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的LAG3含量。LAG3，全称淋巴细胞活化基因3蛋白，是一种免疫球蛋白超家族成员，主要在T细胞上表达。它通过与MHC II类分子结合，抑制T细胞活性，从而在免疫逃逸中发挥作用。研究显示，LAG3的泛素化修饰是其激活的关键，通过阻碍膜结合释放FSALE基序，激活LAG3的抑制功能，为癌症免疫治疗提供了新的靶点。试剂盒检测范围为1.25 ng/mL-80 ng/mL，科研人员可通过定量分析小鼠模型中的LAG3表达水平，广泛应用于肿瘤微环境研究、免疫治疗药物开发、免疫相关疾病动物模型评价等基础研究领域，为探索LAG3在免疫调控网络中的作用提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Lag3 ELISA Kit; Lymphocyte activation gene 3 protein ELISA Kit; LAG-3 ELISA Kit; Activation-induced cytidine deaminase-linked autoimmunity protein ELISA Kit; Aida ELISA Kit; CD antigen CD223) [Cleaved into: Secreted lymphocyte activation gene 3 protein ELISA Kit; sLAG-3)] ELISA Kit
缩写：LAG3
Uniprot No.：Q61790
种属：Mus musculus (Mouse)
样本类型：serum, plasma, tissue homogenates
检测范围：1.25 ng/mL-80 ng/mL
灵敏度：0.31 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse LAG3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:5 | Average % | 93 | ||||
| Range % | 89-97 | |||||
| 1:10 | Average % | 104 | ||||
| Range % | 98-107 | |||||
| 1:20 | Average % | 91 | ||||
| Range % | 84-94 | |||||
| 1:40 | Average % | 99 | ||||
| Range % | 94-102 | |||||
回收率：
| The recovery of mouse LAG3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 90 | 85-95 | ||||
| EDTA plasma (n=4) | 95 | 88-98 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected802.8592.7452.8022.694402.0422.0782.0601.952201.1331.1121.1231.015100.6630.6430.6530.54550.3720.3820.3770.2692.50.2750.2810.2780.1701.250.1840.1780.1810.07300.1090.1070.108 | ||||||
货期：3-5 working days
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