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产品描述：人层粘连蛋白-5（LN-5）酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆样本中的LAMA5含量。LAMA5是层粘连蛋白α5亚基，在细胞黏附、迁移和组织发育等过程发挥重要作用。研究发现它与肿瘤进展密切相关，能调节肿瘤细胞增殖与转移，还参与肿瘤血管生成。目前针对LAMA5作用机制及作为治疗靶点的研究，有望为肿瘤等疾病防治提供新思路。试剂盒检测范围为12.5 ng/mL-800 ng/mL，支持科研人员在体外实验体系中开展LN - 5动态变化研究，例如皮肤修复模型、上皮组织损伤评估或特定病理状态下基底膜代谢异常的机制解析等方向，为细胞外基质相关的基础研究提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：KIAA0533 ELISA Kit; KIAA1907 ELISA Kit; LAMA5 ELISA Kit; LAMA5_HUMAN ELISA Kit; Laminin alpha 5 chain ELISA Kit; Laminin subunit alpha 5 ELISA Kit; Laminin subunit alpha-5 ELISA Kit; Laminin; alpha 5 ELISA Kit; Laminin-10 subunit alpha ELISA Kit; Laminin-11 subunit alpha ELISA Kit; Laminin-15 subunit alpha ELISA Kit; RP11-157P1.6 ELISA Kit
缩写：LAMA5
Uniprot No.：O15230
种属：Homo sapiens (Human)
样本类型：serum, plasma
检测范围：12.5 ng/mL-800 ng/mL
灵敏度：3.12 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Neuroscience
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of Human LN-5 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 88 | ||||
| Range % | 84-94 | |||||
| 1:2 | Average % | 98 | ||||
| Range % | 92-102 | |||||
| 1:4 | Average % | 95 | ||||
| Range % | 90-100 | |||||
| 1:8 | Average % | 102 | ||||
| Range % | 96-106 | |||||
回收率：
| The recovery of Human LN-5 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 94 | 88-98 | ||||
| EDTA plasma (n=4) | 86 | 82-92 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected8002.2622.3682.3152.2164001.6761.5701.6231.5242000.9870.9600.9740.8751000.5580.5470.5530.454500.3120.3240.3180.219250.2430.2560.2500.15112.50.1720.1740.1730.07400.0980.0990.099 | ||||||
货期：3-5 working days
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