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产品描述：小鼠溶酶体相关膜糖蛋白2(LAMP2)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的LAMP2含量。LAMP2是一种重要靶点。它在细胞内吞、自噬等过程发挥关键作用，参与维持细胞内环境稳定。相关研究聚焦其在肿瘤等疾病中表达变化与功能机制，探索其作为生物标志物及治疗靶点的潜力，为开发针对性疗法提供方向。试剂盒检测范围为31.25 pg/mL-2000 pg/mL，适用于基础科研中探索溶酶体相关生理机制、构建疾病模型（如神经退行性疾病或心血管疾病）的分子变化研究，以及药物干预下溶酶体功能评估等实验需求。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Lamp2; Lamp-2; Lysosome-associated membrane glycoprotein 2; LAMP-2; Lysosome-associated membrane protein 2; CD107 antigen-like family member B; Lysosomal membrane glycoprotein type B; LGP-B; CD antigen CD107b
缩写：LAMP2
Uniprot No.：P17047
种属：Mus musculus (Mouse)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：31.25 pg/mL-2000 pg/mL
灵敏度：7.81 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cardiovascular
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse LAMP2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 92 | |||||
| Range % | 87-96 | ||||||
| 1:2 | Average % | 105 | |||||
| Range % | 101-109 | ||||||
| 1:4 | Average % | 86 | |||||
| Range % | 83-89 | ||||||
| 1:8 | Average % | 98 | |||||
| Range % | 94-102 | ||||||
回收率：
| The recovery of mouse LAMP2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 106 | 103-109 | |||||
| EDTA plasma (n=4) | 93 | 90-96 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| pg/mlOD1OD2AverageCorrected20002.4922.5342.5132.37110002.0172.0092.0131.8715001.2971.2041.2511.1092500.6690.6780.6740.5321250.3760.3820.3790.23762.50.2560.2630.2600.11831.250.1920.1870.1900.04800.1410.1430.142 | |||||||
货期：3-5 working days
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