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产品描述：人LIM和SH3结构域蛋白1(LASP1)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的LASP1含量。LASP1 是一种重要的靶点。它在细胞的迁移、增殖等过程中发挥作用。研究表明，LASP1 与肿瘤发生发展密切相关，可能通过调节细胞内信号通路来影响肿瘤细胞的行为，对其深入研究有助于为癌症等疾病的治疗提供新方向。试剂盒检测范围为25 pg/mL-1600 pg/mL，适用于生命科学领域对LASP1表达调控机制的研究，例如肿瘤微环境分析、细胞运动性实验或药物干预效果评估等科研场景本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：LASP 1 ELISA Kit; LASP-1 ELISA Kit; LASP1 ELISA Kit; LASP1_HUMAN ELISA Kit; LIM and SH3 domain protein 1 ELISA Kit; LIM and SH3 protein 1 ELISA Kit; Metastatic lymph node gene 50 protein ELISA Kit; MLN 50 ELISA Kit; MLN50 ELISA Kit; OTTHUMP00000164238 ELISA Kit; OTTHUMP00000164239 ELISA Kit
缩写：LASP1
Uniprot No.：Q14847
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：25 pg/mL-1600 pg/mL
灵敏度：6.25 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human LASP1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 97 | ||||
| Range % | 92-101 | |||||
| 1:2 | Average % | 88 | ||||
| Range % | 83-92 | |||||
| 1:4 | Average % | 97 | ||||
| Range % | 93-101 | |||||
| 1:8 | Average % | 85 | ||||
| Range % | 80-92 | |||||
回收率：
| The recovery of human LASP1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 99 | 95-103 | ||||
| EDTA plasma (n=4) | 91 | 87-95 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected16002.6032.5722.5882.4578002.1572.1062.1322.0014001.6401.5241.5821.4512001.1531.0691.1110.9801000.7140.7420.7280.597500.4190.4430.4310.300250.2490.2710.2600.12900.1270.1350.131 | ||||||
货期：3-5 working days
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