| 纯度 | >95%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SULT1B1 |
| Uniprot No | O43704 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-296aa |
| 氨基酸序列 | MGSSHHHHHH SSGLVPRGSH MGSHMLSPKD ILRKDLKLVH GYPMTCAFAS NWEKIEQFHS RPDDIVIATY PKSGTTWVSE IIDMILNDGD IEKCKRGFIT EKVPMLEMTL PGLRTSGIEQ LEKNPSPRIV KTHLPTDLLP KSFWENNCKM IYLARNAKDV SVSYYHFDLM NNLQPFPGTW EEYLEKFLTG KVAYGSWFTH VKNWWKKKEE HPILFLYYED MKENPKEEIK KIIRFLEKNL NDEILDRIIH HTSFEVMKDN PLVNYTHLPT TVMDHSKSPF MRKGTAGDWK NYFTVAQNEK FDAIYETEMS KTALQFRTEI |
| 预测分子量 | 37 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于SULT1B1重组蛋白的3篇代表性文献示例(注：内容为模拟生成，实际文献需通过数据库检索确认)：
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1. **文献名称**：*"Expression and Characterization of Recombinant Human SULT1B1: Substrate Specificity and Role in Catecholamine Metabolism"*
**作者**：Wang et al. (2005)
**摘要**：本研究在大肠杆菌中成功表达并纯化了人源SULT1B1重组蛋白，证实其对多巴胺、甲状腺激素等底物具有高催化活性，并揭示了其在儿茶酚胺类代谢中的潜在作用。
2. **文献名称**：*"Structural Insights into the Catalytic Mechanism of SULT1B1 through Crystallographic Analysis"*
**作者**：Maiti et al. (2012)
**摘要**：首次解析了SULT1B1重组蛋白的晶体结构，阐明了其与辅因子PAPS(3'-磷酸腺苷-5'-磷酰硫酸)的结合模式及底物识别机制，为设计特异性抑制剂奠定基础。
3. **文献名称**：*"Functional Polymorphisms of SULT1B1 Recombinant Protein and Their Impact on Drug Metabolism"*
**作者**：Nowell et al. (2017)
**摘要**：通过构建不同单核苷酸多态性(SNP)突变的SULT1B1重组蛋白，发现某些突变体(如R160H)显著降低对异黄酮类化合物的磺化活性，提示个体代谢差异的分子机制。
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**建议检索平台**：PubMed、Web of Science(关键词：SULT1B1 recombinant expression/purification/kinetics)。实际研究可能涉及酶动力学、病理关联或药物开发。
**Background of SULT1B1 Recombinant Protein**
Sulfotransferase 1B1 (SULT1B1) is a cytosolic enzyme belonging to the sulfotransferase (SULT) superfamily, which catalyzes the transfer of a sulfonate group from 3’-phosphoadenosine 5’-phosphosulfate (PAPS) to hydroxyl or amine groups of substrates. This post-translational modification plays a critical role in the metabolism and detoxification of endogenous compounds (e.g., hormones, neurotransmitters) and xenobiotics (e.g., drugs, environmental toxins). SULT1B1 is primarily expressed in the gastrointestinal tract, liver, and lymphocytes, with substrate specificity for small phenolic compounds, thyroid hormones, and certain xenobiotics.
Recombinant SULT1B1 protein is produced using heterologous expression systems (e.g., *E. coli* or mammalian cells) to enable functional and structural studies. Its recombinant form retains enzymatic activity, allowing researchers to investigate its kinetic properties, substrate preferences, and inhibition mechanisms *in vitro*. This protein is often purified with affinity tags (e.g., His-tag) for ease of isolation and characterization.
Research on recombinant SULT1B1 has implications for understanding interindividual variability in drug metabolism, as genetic polymorphisms in *SULT1B1* may influence enzymatic activity and susceptibility to toxin-induced diseases. It also serves as a tool for screening potential drug candidates or environmental chemicals for sulfonation capacity, which can affect their bioavailability, toxicity, or half-life. Additionally, structural studies using recombinant SULT1B1 contribute to elucidating substrate-binding domains and catalytic mechanisms, aiding in the design of targeted modulators. Overall, SULT1B1 recombinant protein is a vital resource for advancing pharmacogenomics, toxicology, and precision medicine.
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