首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：大鼠脂多糖结合蛋白(LBP)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、组织匀浆样本中的LBP含量。LBP（局部二值模式）是一种用于描述图像局部纹理特征的算子，具有灰度不变性和旋转不变性等显著优点。它由T. Ojala等人在1994年提出，广泛应用于计算机视觉领域，如人脸识别和目标检测。LBP通过比较中心像素与邻域像素的灰度值，生成二进制编码，反映纹理信息。其原理简单，计算效率高，对光照变化不敏感。试剂盒检测范围为62.5 pg/mL-4000 pg/mL，适用于评估败血症、肠道炎症等病理模型中LBP的动态变化，或用于免疫调节药物开发中生物标志物的定量分析，支持科研人员在体外实验中系统研究LBP在炎症反应、细菌感染等生理病理过程中的作用机制，为相关领域的分子机制探索提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：LbpLipopolysaccharide-binding protein ELISA Kit; LBP ELISA Kit
缩写：LBP
Uniprot No.：Q63313
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma, cell culture supernates, tissue homogenates
检测范围：62.5 pg/mL-4000 pg/mL
灵敏度：15.6 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat LBP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:5 | Average % | 85 | ||||
| Range % | 81-89 | |||||
| 1:10 | Average % | 94 | ||||
| Range % | 91-98 | |||||
| 1:20 | Average % | 91 | ||||
| Range % | 85-97 | |||||
| 1:40 | Average % | 86 | ||||
| Range % | 82-89 | |||||
回收率：
| The recovery of rat LBP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 100 | 97-103 | ||||
| EDTA plasma (n=4) | 94 | 90-98 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected40002.8692.8992.8842.68820002.2312.2682.2502.05410001.5141.5691.5421.3465000.9690.9990.9840.7882500.6280.6480.6380.4421250.4320.4690.4510.25562.50.2970.2780.2880.09200.1970.1950.196 | ||||||
货期：3-5 working days
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