首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人脂多糖结合蛋白(LBP)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的LBP含量。LBP即脂多糖结合蛋白，在天然免疫中发挥重要作用。它能与脂多糖结合并转运，激活免疫细胞反应。研究聚焦于其在感染性疾病发病机制中的作用，也探索以此为靶点开发干预措施，以调节免疫反应、改善病情。试剂盒检测范围为0.625 μg/mL-40 μg/mL，该产品适用于体外科研实验中评估不同处理条件下（如病原体感染、药物干预或炎症模型）生物样本的LBP表达水平变化，为探索感染性休克、肠道屏障功能障碍等病理过程的分子机制提供数据支持。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：BPI fold containing family D, member 2 ELISA Kit; Bpifd2 ELISA Kit; LBP ELISA Kit; LBP_HUMAN ELISA Kit; LBP1 ELISA Kit; Lipopolysaccharide binding protein ELISA Kit; Lipopolysaccharide-binding protein ELISA Kit; LPS binding protein ELISA Kit; Ly88 ELISA Kit; MGC22233 ELISA Kit; OTTHUMP00000030965 ELISA Kit; RP23-407H16.4 ELISA Kit
缩写：LBP
Uniprot No.：P18428
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：0.625 μg/mL-40 μg/mL
灵敏度：0.156 μg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human LBP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 97 | ||||
| Range % | 92-102 | |||||
| 1:2 | Average % | 95 | ||||
| Range % | 91-99 | |||||
| 1:4 | Average % | 99 | ||||
| Range % | 96-108 | |||||
| 1:8 | Average % | 94 | ||||
| Range % | 89-98 | |||||
回收率：
| The recovery of human LBP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 99 | 92-108 | ||||
| EDTA plasma (n=4) | 84 | 80-88 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| μg/mlOD1OD2AverageCorrected402.7952.6942.7452.613202.2322.1312.1822.050101.4641.4431.4541.32250.8120.8230.8180.6862.50.4250.4150.4200.2881.250.3000.3110.3060.1740.6250.2220.2180.2200.08800.1320.1310.132 | ||||||
本试剂盒所含材料：
A micro ELISA plate ---The 96-well plate has been pre-coated with an anti-human LBP antibody. This dismountable microplate can be divided into 12 x 8 strip plates.
Two vials lyophilized standard ---Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
One vial Biotin-labeled LBP antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody.
One vial HRP-avidin (100 x concentrate) (120 μl/bottle) ---Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
One vial Biotin-antibody Diluent (15 ml/bottle) ---Dilute the Biotin-antibody.
One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the HRP-avidin solution.
本试剂盒不含材料：
A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
An incubator can provide stable incubation conditions up to 37°C±5°C.
Centrifuge
Vortex
Squirt bottle, manifold dispenser, or automated microplate washer
Absorbent paper for blotting the microtiter plate
货期：3-5 working days
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