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产品描述：人卵磷脂胆固醇酰基转移酶(LCAT)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的LCAT含量。LCAT（卵磷脂胆固醇酰基转移酶）是一种在肝脏合成的酶，主要作用是将胆固醇和磷脂酰胆碱转化为胆固醇酯和溶血磷脂酰胆碱，参与血浆脂蛋白的代谢和胆固醇逆向转运。LCAT的研究对于理解脂代谢和心血管疾病的发生机制具有重要意义。试剂盒检测范围为3.12 ng/mL-200 ng/mL，适用于基础医学研究中LCAT表达水平检测、脂质代谢相关疾病机制探索、药物干预效果评估等场景，可支持动物模型或体外实验样本的批量检测需求。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：LCAT ELISA Kit; LCAT_HUMAN ELISA Kit; Lecithin cholesterol acyltransferase ELISA Kit; Lecithin-cholesterol acyltransferase ELISA Kit; Phosphatidylcholine sterol acyltransferase ELISA Kit; Phosphatidylcholine-sterol acyltransferase ELISA Kit; Phospholipid cholesterol acyltransferase ELISA Kit; Phospholipid-cholesterol acyltransferase ELISA Kit
缩写：LCAT
Uniprot No.：P04180
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：3.12 ng/mL-200 ng/mL
灵敏度：0.78 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Metabolism
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human LCAT in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:200 | Average % | 87 | ||||
| Range % | 82-95 | |||||
| 1:400 | Average % | 97 | ||||
| Range % | 90-105 | |||||
| 1:800 | Average % | 101 | ||||
| Range % | 92-110 | |||||
| 1:1600 | Average % | 92 | ||||
| Range % | 84-98 | |||||
回收率：
| The recovery of human LCAT spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 95 | 80-98 | ||||
| EDTA plasma (n=4) | 95 | 90-99 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/ml.OD1OD2AverageCorrected2002.8422.7522.7972.7011002.2472.1762.2122.116501.7391.6041.6721.576251.2151.1391.1771.08112.50.8220.8040.8130.7176.250.5160.4990.5080.4123.120.2330.2290.2310.13500.0970.0950.096 | ||||||
货期：3-5 working days
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