| 纯度 | > 95 % SDS-PAGE. |
| 种属 | Human |
| 靶点 | ATG10 |
| Uniprot No | Q9H0Y0 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-220aa |
| 氨基酸序列 | MGSSHHHHHH SSGLVPRGSH MGSMEEDEFI GEKTFQRYCA EFIKHSQQIG DSWEWRPSKD CSDGYMCKIH FQIKNGSVMS HLGASTHGQT CLPMEEAFEL PLDDCEVIET AAASEVIKYE YHVLYSCSYQ VPVLYFRASF LDGRPLTLKD IWEGVHECYK MRLLQGPWDT ITQQEHPILG QPFFVLHPCK TNEFMTPVLK NSQKINKNVN YITSWLSIVG PVVGLNLPLS YAKATSQDER NVP |
| 预测分子量 | 28 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇与ATG10重组蛋白相关的代表性文献摘要：
1. **《Recombinant ATG10 regulates autophagosome formation through LC3 lipidation》**
- 作者：Kirisako T. et al.
- 摘要：研究揭示了ATG10重组蛋白在自噬体形成中的作用，证明其通过促进ATG12-ATG5复合物与LC3的脂化反应，调控自噬过程的分子机制。
2. **《Structural analysis of human ATG10 provides insights into its catalytic mechanism in autophagy》**
- 作者：Mizushima N. et al.
- 摘要：通过X射线晶体学解析ATG10重组蛋白的三维结构，阐明其作为E2泛素样酶催化ATG12与ATG5结合的活性位点及功能域，为靶向自噬的药物设计提供依据。
3. **《ATG10 overexpression suppresses apoptosis in cancer cells by enhancing autophagic flux》**
- 作者：Levine B. et al.
- 摘要：利用重组ATG10蛋白实验，证明其在肿瘤细胞中通过增强自噬流减少线粒体损伤介导的细胞凋亡，提示ATG10可能成为癌症治疗的潜在靶点。
注：以上为模拟摘要，实际文献需通过PubMed或Web of Science检索关键词“ATG10 recombinant protein”获取。
**Background of ATG10 Recombinant Protein**
ATG10 (Autophagy-related protein 10) is a critical enzyme in the autophagy pathway, a conserved cellular process responsible for degrading and recycling damaged organelles, misfolded proteins, and intracellular pathogens. As an E2-like conjugating enzyme, ATG10 facilitates the covalent conjugation of ATG12 to ATG5. a step essential for forming the ATG12-ATG5-ATG16L1 complex. This complex, in turn, promotes the lipidation of microtubule-associated protein 1A/1B-light chain 3 (LC3), enabling autophagosome membrane expansion and cargo sequestration. Dysregulation of ATG10 is linked to various diseases, including cancer, neurodegenerative disorders, and infectious diseases, underscoring its importance in maintaining cellular homeostasis.
Recombinant ATG10 protein is produced using heterologous expression systems (e.g., *E. coli*, mammalian cells) to study its structure, function, and interactions in vitro. Its production typically involves cloning the ATG10 gene into an expression vector, followed by purification via affinity chromatography. The recombinant protein retains enzymatic activity, enabling researchers to dissect molecular mechanisms of autophagy, screen for autophagy-modulating compounds, or develop therapeutic strategies targeting autophagy-related pathologies.
Studies using ATG10 recombinant protein have revealed its role in selective autophagy subtypes (e.g., mitophagy, xenophagy) and its interplay with signaling pathways like mTOR and AMPK. Additionally, ATG10 overexpression or mutations have been observed in cancers, suggesting its potential as a biomarker or therapeutic target. By providing a purified, functional form of ATG10. recombinant protein tools advance both basic research and translational applications in autophagy biology.
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