首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠中性粒细胞明胶酶相关脂质运载蛋白(NGAL)酶联免疫试剂盒为竞争法ELISA试剂盒，定量检测血清、血浆、组织匀浆、尿液样本中的LCN2含量。试剂盒检测范围为12.5 pg/mL-800 pg/mL，本试剂盒采用预包被特异性抗体的微孔板，通过样本中NGAL与生物素标记抗原竞争结合抗体，结合酶标链霉亲和素催化显色反应，实现目标蛋白的高特异性定量检测，适用于小鼠疾病模型（如肾损伤、脓毒症、肿瘤）的机制研究、药物干预效果评价及生物标志物分析。样本兼容性广，涵盖血液、组织及尿液等多种生物基质，为炎症相关科研提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Lcn2 ELISA Kit; Neutrophil gelatinase-associated lipocalin ELISA Kit; NGAL ELISA Kit; Lipocalin-2 ELISA Kit; Oncogene 24p3 ELISA Kit; 24p3 ELISA Kit; SV-40-induced 24p3 protein ELISA Kit; Siderocalin LCN2 ELISA Kit; p25 ELISA Kit
缩写：LCN2
Uniprot No.：P11672
种属：Mus musculus (Mouse)
样本类型：serum, plasma, tissue homogenates, urine
检测范围：12.5 pg/mL-800 pg/mL
灵敏度：3.12 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Competitive
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse NGAL in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:200 | Average % | 105 | |||||
| Range % | 101-108 | ||||||
| 1:400 | Average % | 97 | |||||
| Range % | 95-99 | ||||||
| 1:800 | Average % | 95 | |||||
| Range % | 90-99 | ||||||
| 1:1600 | Average % | 90 | |||||
| Range % | 84-96 | ||||||
回收率：
| The recovery of mouse NGALspiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 90 | 87-93 | |||||
| EDTA plasma (n=4) | 97 | 92-102 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| pg/mlOD1OD2Average8000.1350.1410.1384000.2580.2650.2622000.5010.5250.5131000.9550.9850.970501.5451.5741.560252.0882.1222.10512.52.4572.5882.52302.8632.6982.781 | |||||||
货期：3-5 working days
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