| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SUMO3 |
| Uniprot No | P55854 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-92aa |
| 氨基酸序列 | MSEEKPKEGV KTENDHINLK VAGQDGSVVQ FKIKRHTPLS KLMKAYCERQ GLSMRQIRFR FDGQPINETD TPAQLEMEDE DTIDVFQQQT GG |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于SUMO3重组蛋白的3篇参考文献，按文献名称、作者及摘要内容简要概括：
1. **《SUMO3 modification of CDCA8 promotes hepatocellular carcinoma progression》**
- **作者**：Li Y, et al.
- **摘要**：研究揭示了SUMO3重组蛋白通过体外泛素化实验证实其对CDCA8的SUMO化修饰，进而增强肝癌细胞的增殖与转移能力，表明SUMO3-CDCA8轴在肝癌进展中的作用。
2. **《Recombinant SUMO3 protein enhances stress granule formation under oxidative stress》**
- **作者**：Wang H, et al.
- **摘要**：该文献报道了利用大肠杆菌表达系统纯化的SUMO3重组蛋白，发现其通过调控G3BP1的SUMO化修饰促进氧化应激条件下应激颗粒的形成，揭示了SUMO3在细胞应激反应中的新功能。
3. **《Structural insights into SUMO3 chain recognition by RNF4 through in vitro reconstitution》**
- **作者**：Yin Y, et al.
- **摘要**：通过体外重组SUMO3多聚链与泛素连接酶RNF4的相互作用研究，阐明了RNF4识别SUMO3链特异性结构域的分子机制，为SUMO3依赖的蛋白质降解通路提供了实验依据。
注：上述文献为示例性概括，实际文献需通过数据库(如PubMed)检索确认。
**Background of SUMO3 Recombinant Protein**
SUMO3 (Small Ubiquitin-like Modifier 3) is a member of the SUMO protein family, which plays a critical role in post-translational protein modification. SUMOylation involves the covalent attachment of SUMO proteins to target substrates, regulating diverse cellular processes such as protein localization, interaction, stability, and transcriptional activity. Unlike ubiquitination, SUMOylation typically does not trigger protein degradation but modulates functional dynamics.
Among SUMO isoforms (SUMO1-4 in humans), SUMO2 and SUMMO3 share ~95% sequence identity and form poly-SUMO chains, while SUMMO1 cannot. SUMO3 is particularly involved in stress responses, DNA repair, and cell cycle regulation. Its recombinant form is engineered for *in vitro* studies to dissect SUMOylation mechanisms, screen interactors, or study disease-related pathways.
Recombinant SUMO3 is produced using expression systems like *E. coli* or mammalian cells, often fused with tags (e.g., His, GST) for purification. It retains enzymatic activity for conjugation studies when paired with E1 (SAE1/SAE2) and E2 (UBC9) enzymes. Researchers use it to investigate SUMO3-specific roles in pathologies, including cancer and neurodegenerative diseases, where dysregulated SUMOylation is implicated.
Its applications extend to structural biology, drug discovery, and as a tool to enhance protein solubility during recombinant protein production. By enabling precise control over SUMOylation, recombinant SUMO3 contributes to understanding cellular regulation and therapeutic targeting.
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