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产品描述：牛瘦素(LEP)酶联免疫试剂盒为竞争法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的LEP含量。LEP（瘦素）是一种由脂肪细胞分泌的蛋白质类激素，通过抑制饥饿来调控能量平衡。它通过与其受体结合，在脂肪组织和下丘脑之间建立负反馈机制，调节食欲和能量代谢。LEP的研究涉及其在肥胖、糖尿病、心血管疾病等疾病中的作用，以及作为治疗靶点的潜力。试剂盒检测范围为3.12 ng/mL-50 ng/mL，本产品适用于畜牧业中牛群代谢健康监测、营养干预研究以及肥胖相关分子机制探索，可兼容多种生物样本类型，为实验室提供稳定可靠的检测工具，助力动物生理学、兽医学及农业科学领域的基础研究。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：LEP ELISA Kit; OB ELISA Kit; OBS ELISA Kit; Leptin ELISA Kit; Obesity factor ELISA Kit
缩写：LEP
Uniprot No.：P50595
种属：Bos taurus (Bovine)
样本类型：serum, plasma, tissue homogenates
检测范围：3.12 ng/mL-50 ng/mL
灵敏度：3.12 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Metabolism
测定原理：quantitative
测定方法：Competitive
精密度：
| Intra-assay Precision (Precision within an assay): CV%<15% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<15% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of bovine LEP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 94 | ||||
| Range % | 89-98 | |||||
| 1:2 | Average % | 90 | ||||
| Range % | 87-94 | |||||
| 1:4 | Average % | 89 | ||||
| Range % | 85-94 | |||||
| 1:8 | Average % | 100 | ||||
| Range % | 96-105 | |||||
回收率：
| The recovery of bovine LEP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 93 | 90-96 | ||||
| EDTA plasma (n=4) | 90 | 85-96 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2Average 500.2620.2530.258 250.5200.5120.516 12.50.8310.8770.854 6.251.7161.6681.692 3.122.4392.5192.479 | ||||||
货期：3-5 working days
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