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产品描述：兔子瘦素(LEP)酶联免疫试剂盒为竞争法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的LEP含量。LEP即瘦素，其编码基因是肥胖基因。它由脂肪组织分泌，可作用于下丘脑调节能量代谢和食欲。研究机制上，通过与瘦素受体结合激活信号通路，调控神经肽表达以影响进食行为等，在体重和代谢平衡调节中至关重要。试剂盒检测范围为15.6 pg/mL-1000 pg/mL，该产品适用于科研领域中对兔模型能量代谢机制、脂肪组织功能、肥胖相关疾病机制的研究，尤其适用于评估脂肪细胞分泌活性或药物干预后LEP的动态变化。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：LEP
Uniprot No.：B0KZL0
种属：Oryctolagus cuniculus (Rabbit)
样本类型：serum, plasma, tissue homogenates
检测范围：15.6 pg/mL-1000 pg/mL
灵敏度：3.9 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Metabolism
测定原理：quantitative
测定方法：Competitive
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rabbit LEP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:100 | Average % | 96 | ||||
| Range % | 91-99 | |||||
| 1:200 | Average % | 93 | ||||
| Range % | 88-97 | |||||
| 1:400 | Average % | 85 | ||||
| Range % | 80-89 | |||||
| 1:800 | Average % | 90 | ||||
| Range % | 84-96 | |||||
回收率：
| The recovery of rabbit LEP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 99 | 94-105 | ||||
| EDTA plasma (n=4) | 87 | 83-97 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2Average 10000.0970.0980.098 5000.2670.2710.269 2500.4770.4660.472 1250.7030.6910.697 62.51.1041.0911.098 31.251.4121.4291.421 15.61.6061.7051.656 01.8131.9151.864 | ||||||
货期：3-5 working days
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