首页 / 产品 / ELISA试剂盒 / 其他ELISA试剂盒
产品描述：猪瘦素(LEP)酶联免疫试剂盒为竞争法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的LEP含量。LEP即瘦素，是脂肪组织分泌的一种肽类激素。其背景在于它参与体重调节、能量代谢等生理过程。研究机制方面，它能作用于下丘脑的受体，调控食欲与能量消耗，异常表达或功能障碍可能与肥胖等疾病相关，是肥胖等领域研究热点。试剂盒检测范围为0.25 ng/mL-50 ng/mL，适用于体外实验中对猪能量代谢相关疾病的机制研究、营养状态评估或脂肪组织功能分析等科研领域；适用于实验室条件下探究瘦素在猪模型中的表达调控、代谢关联性及其与内分泌系统的相互作用，为畜牧科学、动物生理学及比较医学研究提供定量分析工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：LEP ELISA Kit; OB ELISA Kit; OBS ELISA Kit; Leptin ELISA Kit; Obesity factor ELISA Kit
缩写：LEP
Uniprot No.：Q29406
种属：Sus scrofa (Pig)
样本类型：serum, plasma, tissue homogenates
检测范围：0.25 ng/mL-50 ng/mL
灵敏度：0.125 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Metabolism
测定原理：quantitative
测定方法：Competitive
精密度：
| Intra-assay Precision (Precision within an assay): CV%<15% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<15% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of pig leptin in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 96 | ||||
| Range % | 91-102 | |||||
| 1:2 | Average % | 92 | ||||
| Range % | 87-98 | |||||
| 1:4 | Average % | 95 | ||||
| Range % | 91-100 | |||||
| 1:8 | Average % | 93 | ||||
| Range % | 84-99 | |||||
回收率：
| The recovery of pig leptin spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 96 | 90-102 | ||||
| EDTA plasma (n=4) | 92 | 88-96 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2Average 02.6652.5292.597 0.251.4611.3871.424 10.9731.0080.991 40.5000.5130.507 12.50.3460.3340.340 500.1500.1550.153 | ||||||
货期：3-5 working days
×
