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产品描述：马瘦素(LEP)酶联免疫试剂盒为竞争法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的LEP含量。LEP（瘦素）是一种由脂肪细胞分泌的蛋白质激素，主要作用是调节能量代谢和食欲抑制。其研究机制涉及与瘦素受体结合，激活下游信号通路，影响进食和能量消耗。LEP的突变或信号传导障碍可能导致肥胖和代谢性疾病。试剂盒检测范围为3.12 pg/mL-200 pg/mL，本试剂盒可广泛应用于马属动物代谢调控机制研究、营养与肥胖相关能量代谢实验、生殖生理学分析，以及疾病模型中激素水平变化的科研探索，为深入解析LEP在马体内的生理病理作用提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：LEP ELISA Kit; OB ELISA Kit; Leptin ELISA Kit; Obesity factor ELISA Kit; Fragment ELISA Kit
缩写：LEP
Uniprot No.：Q9TU09
种属：Equus caballus (Horse)
样本类型：serum, plasma, tissue homogenates
检测范围：3.12 pg/mL-200 pg/mL
灵敏度：0.78 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Metabolism
测定原理：quantitative
测定方法：Competitive
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of horse LEP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:100 | Average % | 99 | ||||
| Range % | 95-104 | |||||
| 1:200 | Average % | 103 | ||||
| Range % | 98-109 | |||||
| 1:400 | Average % | 89 | ||||
| Range % | 84-95 | |||||
| 1:800 | Average % | 96 | ||||
| Range % | 91-101 | |||||
回收率：
| The recovery of horse LEP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 93 | 86-100 | ||||
| EDTA plasma (n=4) | 104 | 100-108 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2Average 2000.0540.0560.055 1000.0940.0990.097 500.1810.1830.182 250.3100.3020.306 12.50.5940.5460.570 6.250.9950.9850.990 3.121.3301.3461.338 01.6841.6051.645 | ||||||
货期：3-5 working days
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