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产品描述：鱼(鲑鱼)瘦素(LEP)酶联免疫试剂盒为竞争法ELISA试剂盒，定量检测血清、血浆样本中的LEP含量。LEP即瘦素基因编码蛋白瘦素，其在能量平衡和体重调控中扮演关键角色。研究机制主要是，瘦素能作用于下丘脑，通过信号传导调节食欲、能量消耗等。当下，围绕LEP在治疗肥胖、代谢性疾病等方面的研究不断开展，以发掘其更多治疗潜力。试剂盒检测范围为62.5 pg/mL-1000 pg/mL，适用于鱼类生理学、营养学及环境毒理学研究，可用于探究饵料成分、温度变化或污染物暴露对鱼类代谢调控的影响，为水产养殖优化和生态保护研究提供可靠工具本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：LEP
Uniprot No.：D6BP01
种属：Fish(Salmon)
样本类型：serum, plasma
检测范围：62.5 pg/mL-1000 pg/mL
灵敏度：31.25 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Metabolism
测定原理：quantitative
测定方法：Competitive
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of fish LEP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:20 | Average % | 91 | ||||
| Range % | 86-98 | |||||
| 1:40 | Average % | 97 | ||||
| Range % | 91-102 | |||||
| 1:80 | Average % | 84 | ||||
| Range % | 80-87 | |||||
| 1:160 | Average % | 90 | ||||
| Range % | 83-96 | |||||
回收率：
| The recovery of fish LEP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 91 | 87-96 | ||||
| EDTA plasma (n=4) | 97 | 93-102 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2Average 10000.0820.0860.084 5000.1900.2000.195 2500.4830.4440.464 1250.9450.9590.952 62.51.6721.5551.614 02.5912.6702.631 | ||||||
货期：3-5 working days
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