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Chicken Leptin,LEP ELISA kit

  • 中文名: 鸡瘦素(LEP)ELISA kit
货号: IPD-E14082C
Price: ¥3000
数量:
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产品详情

  • 产品描述:鸡瘦素(LEP)酶联免疫试剂盒为双抗夹心法ELISA试剂盒,定量检测血清、血浆、组织匀浆样本中的LEP含量。LEP(瘦素)是一种由脂肪细胞分泌的蛋白质类激素,通过抑制饥饿来调控能量平衡。其研究机制涉及与瘦素受体结合,激活下游信号通路,抑制进食并促进能量消耗。LEP的研究对肥胖、糖尿病等代谢性疾病有重要意义。试剂盒检测范围为0.312 ng/mL-20 ng/mL,科研人员可通过本试剂盒开展家禽生长调控机制、脂肪代谢通路、繁殖生理学以及营养干预等基础研究,为禽类健康管理及遗传育种提供实验依据。本品仅用于科研,不用于临床诊断,产品具体参数及操作步骤详见产品说明书。

  • 别名:LEP ELISA Kit; OB ELISA Kit; Leptin ELISA Kit; Obesity factor ELISA Kit

  • 缩写:LEP

  • Uniprot No.:O42164

  • 种属:Gallus gallus (Chicken)

  • 样本类型:serum, plasma, tissue homogenates

  • 检测范围:0.312 ng/mL-20 ng/mL

  • 灵敏度:0.078 ng/mL

  • 反应时间:1-5h

  • 样本体积:50-100ul

  • 检测波长:450 nm

  • 研究领域:Metabolism

  • 测定原理:quantitative

  • 测定方法:Sandwich

  • 精密度:

    Intra-assay Precision (Precision within an assay): CV%<8%


    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%


    Three samples of known concentration were tested in twenty assays to assess.








  • 线性度:

    To assess the linearity of the assay, samples were spiked with high concentrations of chicken LEP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

    SampleSerum(n=4)
    1:1Average %92
    Range %86-101
    1:2Average %95
    Range %85-104
    1:4Average %91
    Range %87-100
    1:8Average %93
    Range %89-106
  • 回收率:

    The recovery of chicken LEP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5)9786-107
    EDTA plasma (n=4)9588-99














  • 标准曲线:

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/mlOD1OD2AverageCorrected202.5402.4722.5062.373101.8791.8991.8891.75651.1381.1231.1310.9982.50.7310.7170.7240.5911.250.4110.4020.4070.2740.6250.3080.3150.3120.1790.3120.2000.2090.2050.07200.1310.1350.133






  • 货期:3-5 working days

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