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产品描述：山羊瘦素(LEP)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆样本中的LEP含量。LEP即瘦素，其背景在于它由脂肪组织分泌，能参与能量代谢与体重调节。研究机制方面，主要是通过与下丘脑的特异性受体结合，抑制食欲、增加能量消耗，在肥胖、代谢紊乱等研究中是重要靶点，为相关疾病治疗提供思路。试剂盒检测范围为0.625 ng/mL-40 ng/mL，适用于科研场景下对山羊能量代谢状态的评估，例如探究营养干预、能量负平衡对脂肪组织功能的影响，或用于建立代谢相关疾病动物模型时的生物标志物分析。本产品为研究山羊肥胖机制、繁殖性能与代谢调控的关联性提供可靠工具本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：LEP ELISA Kit; OBLeptin ELISA Kit; Obesity factor ELISA Kit
缩写：LEP
Uniprot No.：Q257X2
种属：Capra hircus (Goat)
样本类型：serum, plasma
检测范围：0.625 ng/mL-40 ng/mL
灵敏度：0.156 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Metabolism
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of goat LEP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 89 | ||||
| Range % | 82-97 | |||||
| 1:2 | Average % | 91 | ||||
| Range % | 85-99 | |||||
| 1:4 | Average % | 92 | ||||
| Range % | 86-100 | |||||
| 1:8 | Average % | 94 | ||||
| Range % | 84-98 | |||||
回收率：
| The recovery of goat LEP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 96 | 90-99 | ||||
| EDTA plasma (n=4) | 93 | 85-101 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected401.9601.9341.9471.805201.7421.7951.7691.627101.4251.3971.4111.26950.9831.0030.9930.8512.50.6550.6490.6520.5101.250.4310.4240.4280.2860.6250.2480.2390.2440.10200.1410.1430.142 | ||||||
货期：3-5 working days
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