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产品描述：绵羊瘦素(LEP)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的LEP含量。LEP即瘦素，是脂肪细胞分泌的肽类激素。用于调节能量代谢和体重。它能作用于下丘脑的受体，抑制食欲、增加能量消耗。此外，还参与调节脂肪代谢、糖代谢等，对维持机体能量平衡起关键作用。试剂盒检测范围为3.12 ng/mL-200 ng/mL，适用于绵羊模型在营养代谢研究、生殖生理学分析、肥胖或能量代谢异常机制探索等科研场景中的LEP水平定量分析，为畜牧业动物健康调控及基础医学研究提供可靠工具，支持科研人员高效完成样本中LEP的动态监测与数据比对，所有检测结果仅用于科学研究用途。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：LEP ELISA Kit; OB ELISA Kit; Leptin ELISA Kit; Obesity factor ELISA Kit
缩写：LEP
Uniprot No.：Q28603
种属：Ovis aries (Sheep)
样本类型：serum, plasma, tissue homogenates
检测范围：3.12 ng/mL-200 ng/mL
灵敏度：0.78 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Metabolism
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of sheep LEP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 90 | ||||
| Range % | 82-98 | |||||
| 1:2 | Average % | 93 | ||||
| Range % | 86-100 | |||||
| 1:4 | Average % | 95 | ||||
| Range % | 88-102 | |||||
| 1:8 | Average % | 95 | ||||
| Range % | 90-100 | |||||
回收率：
| The recovery of sheep LEP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 92 | 88-96 | ||||
| EDTA plasma (n=4) | 95 | 90-101 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected2002.1492.2472.1982.1051001.1951.1311.1631.070500.6080.5850.5970.504250.3470.3260.3370.24412.50.2140.2240.2190.1266.250.1660.1550.1610.0683.120.1380.1430.1410.04800.0940.0920.093 | ||||||
货期：3-5 working days
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