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产品描述：人乳糖凝集素1(LGALS1)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的LGALS1含量。LGALS1（半乳糖凝集素-1）是一种由LGALS1基因编码的蛋白质，属于半乳糖凝集素家族。它在多种细胞和组织中存在，参与细胞黏附、增殖、凋亡和炎症反应等生理过程。LGALS1在母胎界面表达丰富，对滋养细胞的增殖、分化、侵袭和子宫内膜容受性的建立有重要作用，同时还能促进血管生成和调节母胎免疫耐受。试剂盒检测范围为3.12 ng/mL-200 ng/mL，该产品适用于肿瘤生物学、免疫调控机制及神经退行性疾病等领域的科研探索，例如用于研究肿瘤微环境中LGALS1与免疫细胞互作的关系，或解析其在神经炎症中的分子调控网络本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：14 kDa laminin-binding protein ELISA Kit; 14 kDa lectin ELISA Kit; Beta galactoside binding lectin ELISA Kit; Beta galactoside binding lectin L 14 I ELISA Kit; beta galactoside binding protein ELISA Kit; Beta-galactoside-binding lectin L-14-I ELISA Kit; DKFZp686E23103 ELISA Kit; Gal 1 ELISA Kit; Gal-1 ELISA Kit; GAL1 ELISA Kit; Galaptin ELISA Kit; Galbp ELISA Kit; Galectin ELISA Kit; Galectin-1 ELISA Kit; Galectin1 ELISA Kit; GBP ELISA Kit; HBL ELISA Kit; HLBP14 ELISA Kit; HPL ELISA Kit; L 14.5 ELISA Kit; L-14.5 ELISA Kit; L14 ELISA Kit; Lactose binding lectin 1 ELISA Kit; Lactose-binding lectin 1 ELISA Kit; Lect14 ELISA Kit; Lectin galactoside binding soluble 1 ELISA Kit; Lectin galactoside-binding soluble 1 ELISA Kit; LEG1_HUMAN ELISA Kit; LGALS 1 ELISA Kit; LGALS1 ELISA Kit; Lgals1 lectin galactose binding soluble 1 ELISA Kit; MAPK activating protein MP12 ELISA Kit; Putative MAPK activating protein MP12 ELISA Kit; Putative MAPK-activating protein PM12 ELISA Kit; S Lac lectin 1 ELISA Kit; S-Lac lectin 1 ELISA Kit
缩写：LGALS1
Uniprot No.：P09382
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：3.12 ng/mL-200 ng/mL
灵敏度：0.78 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cell Biology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human LGALS1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 86 | ||||
| Range % | 80-94 | |||||
| 1:2 | Average % | 85 | ||||
| Range % | 80-92 | |||||
| 1:4 | Average % | 92 | ||||
| Range % | 87-99 | |||||
| 1:8 | Average % | 97 | ||||
| Range % | 90-105 | |||||
回收率：
| The recovery of human LGALS1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 90 | 83-98 | ||||
| EDTA plasma (n=4) | 94 | 88-100 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected 2002.3122.1432.2282.074 1001.9531.8231.8881.734 501.5621.4821.5221.368 251.1981.1341.1661.012 12.50.6470.5960.6220.468 6.250.3710.3470.3590.205 3.120.2340.2190.2270.073 00.1580.1490.154 | ||||||
货期：3-5 working days
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