首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠半乳糖凝集素-3(Gal-3)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的LGALS3含量。LGALS3 是一个重要靶点。它在细胞黏附、增殖、凋亡等多种生理病理过程中发挥作用。相关研究围绕其与肿瘤发生发展、免疫调节等机制展开，探索通过抑制其活性来开发新型治疗药物，为癌症等疾病的治疗带来新希望。试剂盒检测范围为0.312 ng/mL-20 ng/mL，适用于小鼠疾病模型构建、药物干预研究及分子机制探索等科研领域，特别针对心血管疾病、肝纤维化或肿瘤微环境研究中Gal - 3的动态变化监测本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Lgals3 ELISA Kit; Galectin-3 ELISA Kit; Gal-3 ELISA Kit; 35 kDa lectin ELISA Kit; Carbohydrate-binding protein 35 ELISA Kit; CBP 35 ELISA Kit; Galactose-specific lectin 3 ELISA Kit; IgE-binding protein ELISA Kit; L-34 galactoside-binding lectin ELISA Kit; Laminin-binding protein ELISA Kit; Lectin L-29 ELISA Kit; Mac-2 antigen ELISA Kit
缩写：LGALS3
Uniprot No.：P16110
种属：Mus musculus (Mouse)
样本类型：serum, plasma, tissue homogenates
检测范围：0.312 ng/mL-20 ng/mL
灵敏度：0.078 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cell Biology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse GAL-3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 91 | ||||
| Range % | 85-103 | |||||
| 1:2 | Average % | 90 | ||||
| Range % | 82-99 | |||||
| 1:4 | Average % | 92 | ||||
| Range % | 86-100 | |||||
| 1:8 | Average % | 92 | ||||
| Range % | 89-103 | |||||
回收率：
| The recovery of mouse GAL-3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 96 | 90-101 | ||||
| EDTA plasma (n=4) | 97 | 91-105 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected 202.8522.8422.8472.703 102.2072.2802.2442.100 51.3571.3841.3711.227 2.50.8410.8620.8520.708 1.250.4750.4580.4670.323 0.6250.2830.2970.2900.146 0.3120.2310.2440.2380.094 00.1420.1460.144 | ||||||
货期：3-5 working days
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