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Mouse Galectin-1(LGALS1) ELISA kit

  • 中文名： 小鼠半乳糖凝集素-1(LGALS1)酶联免疫试剂盒
货号: IPD-EL012882MO
Price： ￥2500
数量：
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产品详情

  • 产品描述：
    小鼠半乳糖凝集素-1(LGALS1)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆,组织匀浆样本中的LGALS1含量。LGALS1是一个具有研究价值的靶点。它在细胞黏附、增殖、凋亡等多种生理病理过程中发挥作用。其相关研究机制可能涉及与特定配体相互作用，影响细胞信号通路。深入研究它有助于揭示疾病发病机制，为开发针对性治疗药物提供新思路。试剂盒检测范围为0.312 ng/mL-20 ng/mL，适用于体外培养细胞、实验动物模型等科研场景中LGALS1蛋白表达的动态分析，如探究肿瘤微环境调控机制、神经炎症相关信号通路研究或免疫调节功能评估；适用于基础医学、分子生物学等领域中LGALS1相关机制研究的定量分析需求本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
  • 别名：
    Lgals1 ELISA Kit; Gbp ELISA Kit; Galectin-1 ELISA Kit; Gal-1 ELISA Kit; 14 kDa lectin ELISA Kit; Beta-galactoside-binding lectin L-14-I ELISA Kit; Galaptin ELISA Kit; Lactose-binding lectin 1 ELISA Kit; Lectin galactoside-binding soluble 1 ELISA Kit; S-Lac lectin 1 ELISA Kit
  • 缩写：
    LGALS1
  • Uniprot No.：
    P16045
  • 种属：
    Mus musculus (Mouse)
  • 样本类型：
    serum, plasma,tissue homogenates
  • 检测范围：
    0.312 ng/mL-20 ng/mL
  • 灵敏度：
    0.078 ng/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Cell Biology
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：

    Intra-assay Precision (Precision within an assay): CV%<8%




    Three samples of known concentration were tested twenty times on one plate to assess.


    Inter-assay Precision (Precision between assays): CV%<10%




    Three samples of known concentration were tested in twenty assays to assess.



  • 线性度：

    To assess the linearity of the assay, samples were spiked with high concentrations of mouse LGALS1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.


    Sample

    Serum(n=4)


    1:1

    Average %

    89


    Range %

    85-95


    1:2

    Average %

    96


    Range %

    89-103


    1:4

    Average %

    97


    Range %

    92-103


    1:8

    Average %

    92


    Range %

    87-97


  • 回收率：

    The recovery of mouse LGALS1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

    Sample Type

    Average % Recovery

    Range


    Serum (n=5)

    96

    90-101


    EDTA plasma (n=4)

    89

    85-95


  • 标准曲线：

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.







    ng/ml

    OD1

    OD2

    Average

    Corrected


    20

    2.629

    2.764

    2.696

    2.535


    10

    2.201

    2.167

    2.184

    2.023


    5

    1.685

    1.628

    1.656

    1.495


    2.5

    1.122

    1.139

    1.130

    0.969


    1.25

    0.624

    0.641

    0.632

    0.471


    0.625

    0.387

    0.373

    0.380

    0.219


    0.312

    0.248

    0.254

    0.251

    0.090


    0

    0.157

    0.165

    0.161




  • 货期：
    3-5 working days

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