| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SYCE3 |
| Uniprot No | A1L190 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-88aa |
| 氨基酸序列 | MGSSHHHHHH SSGLVPRGSH MDDADPEERN YDNMLKMLSD LNKDLEKLLE EMEKISVQAT WMAYDMVVMR TNPTLAESMR RLEDAFVNCK EEMEKNWQEL LHETKQRL |
| 预测分子量 | 13 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于SYCE3重组蛋白的3篇参考文献概览：
---
1. **文献名称**：*SYCE3 is required for synaptonemal complex assembly and chiasma formation in mammalian meiosis*
**作者**：Schramm S, et al.
**摘要**：该研究通过小鼠模型发现SYCE3是联会复合体组装的关键因子，其缺失导致减数分裂染色体配对失败和交叉重组缺陷，证实SYCE3在生殖细胞发育中不可或缺。
---
2. **文献名称**：*Structural analysis of SYCE3 interaction with SYCE1/TERB1 in the synaptonemal complex*
**作者**：Gómez-H L, et al.
**摘要**：通过X射线晶体学解析SYCE3与SYCE1的复合结构，揭示其通过特定结构域介导联会复合体横向纤维的连接，为减数分裂染色体稳定性提供分子机制支持。
---
3. **文献名称**：*Recombinant SYCE3 production and functional characterization in homologous recombination assays*
**作者**：Wang Y, et al.
**摘要**：报道在大肠杆菌中高效表达纯化SYCE3重组蛋白，体外实验证明其促进DNA链配对和重组酶活性，提示SYCE3可能在同源重组修复中具有直接作用。
---
(注：以上为模拟文献，实际研究中请通过PubMed/Google Scholar以“SYCE3 recombination protein”等关键词检索最新论文。)
SYCE3 (Synaptonemal Complex Central Element protein 3) is a crucial component of the synaptonemal complex (SC), a proteinaceous structure essential for meiosis in sexually reproducing organisms. The SC mediates the pairing, alignment, and recombination of homologous chromosomes during prophase I, ensuring accurate genetic exchange and chromosome segregation. SYCE3 localizes to the central region of the SC, where it interacts with other central element proteins (e.g., SYCE1. SYCE2. TEX12) to stabilize the architecture of the SC and facilitate crossover formation. Studies in mice and humans have demonstrated that SYCE3 deficiency disrupts SC assembly, leading to meiotic arrest, gametogenesis failure, and infertility.
Recombinant SYCE3 protein is engineered using heterologous expression systems (e.g., *E. coli*, mammalian cells) for functional and structural studies. Its production enables researchers to investigate SYCE3's role in SC dynamics, its interactions with binding partners, and its contribution to meiotic recombination. For example, biochemical assays using recombinant SYCE3 have revealed its ability to form filamentous structures *in vitro*, mimicking its in vivo role in SC assembly. Additionally, mutations or dysregulation of SYCE3 have been linked to reproductive disorders, making the recombinant protein a valuable tool for exploring molecular mechanisms underlying infertility and potential therapeutic targets. Current research also focuses on how SYCE3 coordinates with other SC proteins to ensure genomic stability, highlighting its broader implications in developmental biology and genetic disease modeling.
×
