| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | TAF10 |
| Uniprot No | Q12962 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 84-218aa |
| 氨基酸序列 | MGSSHHHHHH SSGLVPRGSH MGSGAAPPEG AISNGVYVLP SAANGDVKPV VSSTPLVDFL MQLEDYTPTI PDAVTGYYLN RAGFEASDPR IIRLISLAAQ KFISDIANDA LQHCKMKGTA SGSSRSKSKD RKYTLTMEDL TPALSEYGIN VKKPHYFT |
| 预测分子量 | 17 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇关于TAF10重组蛋白的参考文献及其摘要概括：
1. **文献名称**：*TAF10 interacts with the transcriptional activation domains of vitamin D receptor-interacting protein 150 and regulates vitamin D3-dependent transcription*
**作者**：Soutoglou, E., et al.
**摘要**：研究通过重组TAF10蛋白与维生素D受体复合体的相互作用，揭示TAF10在维生素D3信号通路中的调控作用，证实其通过结合转录激活域影响靶基因表达。
2. **文献名称**：*Reconstitution of the human TFIID from recombinant polypeptides: TAF10 is required for transcriptional activation*
**作者**：Gangloff, Y.G., et al.
**摘要**：利用重组TAF10蛋白与其他TFIID亚基共表达，成功在体外重构功能性TFIID复合体，证明TAF10对RNA聚合酶II介导的转录激活不可或缺。
3. **文献名称**：*Structure and function of the histone-fold in the TAF10-TAF12 heterodimer*
**作者**：Birck, C., et al.
**摘要**：通过X射线晶体学解析重组TAF10-TAF12异源二聚体的结构，揭示其组蛋白折叠域在TFIID复合体组装中的关键作用，并阐明其与DNA结合的分子机制。
4. **文献名称**：*Drosophila TAF10 is required for developmental progression and regulates specific transcription factors*
**作者**：Chen, X., et al.
**摘要**：利用重组TAF10蛋白进行果蝇模型研究，发现TAF10缺失导致胚胎发育停滞，并通过调控特定转录因子(如dTAF6)影响下游基因网络。
以上文献均涉及TAF10重组蛋白在转录复合体组装、结构解析及功能调控方面的研究。
TAF10 (TATA-box binding protein-associated factor 10) is a critical subunit of the TFIID complex, a multisubunit transcription factor essential for RNA polymerase II-mediated gene transcription. As part of the TFIID complex, TAF10 interacts with TATA-binding protein (TBP) and other TAFs to nucleate the assembly of the pre-initiation complex at gene promoters. It plays a dual role in transcription regulation: facilitating the recognition of core promoter elements and serving as a coactivator by mediating interactions with gene-specific activators. Structurally, TAF10 contains conserved histone-fold domains that enable dimerization with other TAFs, contributing to the stability and functional integrity of TFIID.
Recombinant TAF10 protein is engineered for in vitro studies to dissect its molecular interactions and mechanisms. Produced via heterologous expression systems (e.g., *E. coli* or insect cells), it retains the ability to integrate into the TFIID complex and participate in transcription assays. Researchers leverage recombinant TAF10 to explore its role in chromatin remodeling, epigenetic regulation, and tissue-specific gene expression. Its dysregulation has been linked to diseases such as cancer, where altered TFIID activity impacts oncogene activation or tumor suppressor silencing. Additionally, TAF10 is implicated in embryogenesis and cell differentiation, making its recombinant form valuable for developmental biology studies. The protein’s solubility and stability in purified form enable structural analyses (e.g., crystallography, cryo-EM) to resolve TFIID architecture and dynamics. By serving as a tool for reconstituting transcription machinery, recombinant TAF10 advances mechanistic insights into transcriptional dysregulation and therapeutic targeting.
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