| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SYT4 |
| Uniprot No | Q9H2B2 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 38-425aa |
| 氨基酸序列 | MGSSHHHHHH SSGLVPRGSH MCQRKSSKSN KTPPYKFVHV LKGVDIYPEN LNSKKKFGAD DKNEVKNKPA VPKNSLHLDL EKRDLNGNFP KTNLKPGSPS DLENATPKLF LEGEKESVSP ESLKSSTSLT SEEKQEKLGT LFFSLEYNFE RKAFVVNIKE ARGLPAMDEQ SMTSDPYIKM TILPEKKHKV KTRVLRKTLD PAFDETFTFY GIPYTQIQEL ALHFTILSFD RFSRDDIIGE VLIPLSGIEL SEGKMLMNRE IIKRNVRKSS GRGELLISLC YQSTTNTLTV VVLKARHLPK SDVSGLSDPY VKVNLYHAKK RISKKKTHVK KCTPNAVFNE LFVFDIPCEG LEDISVEFLV LDSERGSRNE VIGQLVLGAA AEGTGGEHWK EICDYPRRQI AKWHVLCDG |
| 预测分子量 | 46 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于SYT4(Synaptotagmin 4)重组蛋白的3篇参考文献示例：
1. **文献名称**: *Synaptotagmin IV modulates synaptic function and long-term potentiation by regulating BDNF release*
**作者**: Wang et al.
**摘要**: 该研究通过重组SYT4蛋白体外实验，证明其通过钙依赖性方式调控脑源性神经营养因子(BDNF)的释放，进而影响海马神经元突触可塑性与长时程增强(LTP)。
2. **文献名称**: *Structural and functional analysis of Syt4 in secretory vesicle exocytosis*
**作者**: Zhang & Chen
**摘要**: 利用重组SYT4蛋白进行结构解析和体外结合实验，发现其C2结构域具有独特的脂质结合特性，并揭示了其在胰岛素分泌囊泡胞吐中的调控机制。
3. **文献名称**: *Syt4 knockout and rescue with recombinant protein reveals its role in gliotransmission*
**作者**: Lee et al.
**摘要**: 通过重组SYT4蛋白回补实验，证实其在星形胶质细胞中调控ATP的钙依赖性释放，影响神经元-胶质细胞间的信号传递。
(注：以上为模拟文献，若需真实文献，建议在PubMed/Google Scholar检索关键词“Synaptotagmin 4 recombinant protein”。)
**Background of SYT4 Recombinant Protein**
Synaptotagmin-4 (SYT4), a member of the synaptotagmin family, is a calcium-binding protein implicated in regulating membrane trafficking and calcium-dependent signaling. The synaptotagmin family, comprising 17 isoforms in humans, is characterized by tandem C2 domains (C2A and C2B) that mediate calcium-sensitive interactions with membranes. While SYT1. the most studied isoform, is a key calcium sensor for synaptic vesicle exocytosis, SYT4 exhibits distinct functional properties, including lower calcium sensitivity and involvement in non-neuronal secretory pathways.
SYT4 is widely expressed in the brain, endocrine cells, and other tissues, where it modulates processes such as asynchronous neurotransmitter release, lysosomal exocytosis, and plasma membrane repair. Studies suggest its role in neuroprotection, synaptic plasticity, and adaptive responses to cellular stress. Dysregulation of SYT4 has been linked to neurodegenerative diseases (e.g., Alzheimer’s), cancer progression, and metabolic disorders, highlighting its therapeutic potential. For instance, SYT4 overexpression in certain cancers correlates with enhanced secretory activity and tumor survival.
Recombinant SYT4 protein is typically produced using bacterial (e.g., *E. coli*) or mammalian expression systems, often fused with tags like His-tag or GST for purification. This engineered protein retains calcium-dependent lipid-binding activity, enabling *in vitro* studies on membrane interaction kinetics, structural analysis, and drug screening.
Research utilizing SYT4 recombinant protein advances understanding of calcium-regulated exocytosis, membrane dynamics, and disease mechanisms. It also serves as a critical tool for developing targeted therapies and biomarkers for SYT4-associated pathologies. Its unique functional profile continues to intrigue researchers exploring cell biology and translational medicine.
(Word count: 297)
×
