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产品描述:小鼠半乳糖凝集素3结合蛋白(LGALS3BP)酶联免疫试剂盒为双抗夹心法ELISA试剂盒,定量检测血清、血浆、组织匀浆样本中的LGALS3BP含量。LGALS3BP是重要的靶点。它在多种疾病尤其肿瘤进程里扮演关键角色,和肿瘤细胞增殖、转移及免疫逃逸相关。研究机制方面,主要聚焦其分子结构与功能,以及和其他生物分子的相互作用,旨在开发针对性治疗药物。试剂盒检测范围为0.39 ng/mL-25 ng/mL,适用于基础研究领域,如探索LGALS3BP在免疫相关疾病模型中的表达变化、评估其在炎症信号通路中的作用机制,或作为肿瘤微环境研究的潜在分子标记物检测工具。本品仅用于科研,不用于临床诊断,产品具体参数及操作步骤详见产品说明书。
别名:Lgals3bp ELISA Kit; Cycap ELISA Kit; MamaGalectin-3-binding protein ELISA Kit; Cyp-C-associated protein ELISA Kit; CyCAP ELISA Kit; Lectin galactoside-binding soluble 3-binding protein ELISA Kit; Protein MAMA ELISA Kit
缩写:LGALS3BP
Uniprot No.:Q07797
种属:Mus musculus (Mouse)
样本类型:serum, plasma, tissue homogenates
检测范围:0.39 ng/mL-25 ng/mL
灵敏度:0.09 ng/mL
反应时间:1-5h
样本体积:50-100ul
检测波长:450 nm
研究领域:Cell Biology
测定原理:quantitative
测定方法:Sandwich
精密度:
Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
Inter-assay Precision (Precision between assays): CV%<10% | |||||||
Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度:
To assess the linearity of the assay, samples were spiked with high concentrations of mouse LGALS3BP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
Sample | Serum(n=4) | ||||||
1:1000 | Average % | 94 | |||||
Range % | 90-98 | ||||||
1:2000 | Average % | 102 | |||||
Range % | 96-107 | ||||||
1:4000 | Average % | 87 | |||||
Range % | 81-92 | ||||||
1:8000 | Average % | 92 | |||||
Range % | 88-96 |
回收率:
The recovery of mouse LGALS3BP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
Sample Type | Average % Recovery | Range | |||||
Serum (n=5) | 102 | 97-107 | |||||
EDTA plasma (n=4) | 98 | 92-103 | |||||
标准曲线:
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
ng/mlOD1OD2AverageCorrected252.8672.7232.7952.70712.51.9881.8561.9221.8346.251.2581.2191.2391.1513.120.7340.7150.7250.6371.560.4680.4790.4740.3860.780.2420.2480.2450.1570.390.1520.1590.1560.06800.0880.0870.088 |
货期:3-5 working days
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