首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述:小鼠肌红蛋白(MYO/MB)酶联免疫试剂盒为双抗夹心法ELISA试剂盒,定量检测血清、血浆、组织匀浆样本中的MB含量。试剂盒检测范围为15.6 ng/mL-1000 ng/mL,适用于运动医学中肌肉损伤模型评估、心血管疾病研究中的心肌组织分析,以及肌肉退行性疾病的基础实验本品仅用于科研,不用于临床诊断,产品具体参数及操作步骤详见产品说明书。
别名:Mb ELISA Kit; Myoglobin ELISA Kit
缩写:MB
Uniprot No.:P04247
种属:Mus musculus (Mouse)
样本类型:serum, plasma, tissue homogenates
检测范围:15.6 ng/mL-1000 ng/mL
灵敏度:3.9 ng/mL
反应时间:1-5h
样本体积:50-100ul
检测波长:450 nm
研究领域:Metabolism
测定原理:quantitative
测定方法:Sandwich
精密度:
Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
Inter-assay Precision (Precision between assays): CV%<10% | |||||||
Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度:
To assess the linearity of the assay, samples were spiked with high concentrations of mouse MYO/MB in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
Sample | Serum(n=4) | ||||||
1:1 | Average % | 87 | |||||
Range % | 83-94 | ||||||
1:2 | Average % | 98 | |||||
Range % | 94-101 | ||||||
1:4 | Average % | 102 | |||||
Range % | 98-105 | ||||||
1:8 | Average % | 95 | |||||
Range % | 90-101 |
回收率:
The recovery of mouse MYO/MB spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
Sample Type | Average % Recovery | Range | |||||
Serum (n=5) | 91 | 85-105 | |||||
EDTA plasma (n=4) | 104 | 88-109 | |||||
标准曲线:
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
ng/mlOD1OD2AverageCorrected10002.5112.6222.5672.3875002.1062.1212.1141.9342501.7151.7231.7191.5391251.1771.2321.2051.02562.50.8090.8210.8150.63531.20.5440.5240.5340.35415.60.3720.3770.3750.19500.1790.1810.180 |
货期:3-5 working days
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