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产品描述：人丝氨酸肽酶2甘露聚糖结合凝集素(MASP2)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、脑脊液（CSF）样本中的MASP2含量。MASP2（甘露糖结合凝集素相关丝氨酸蛋白酶 2）在补体系统激活中发挥关键作用。其异常激活与多种疾病相关，如非典型溶血尿毒综合征等。研究机制主要聚焦于抑制 MASP2 活性来阻断补体系统过度激活，为相关疾病的治疗提供新思路。试剂盒检测范围为23.44 pg/mL-1500 pg/mL，适用于体外研究补体系统活化机制、评估炎症相关疾病模型中的MASP2动态变化，或探索神经炎症模型中脑脊液生物标志物表达特征。实验操作兼容多种生物样本类型，为免疫学、分子病理学等基础研究提供可靠工具，尤其适合需要微量检测的细胞因子调控研究或体液样本中低丰度蛋白分析。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：MASP2Mannan-binding lectin serine protease 2 ELISA Kit; EC 3.4.21.104 ELISA Kit; MBL-associated serine protease 2 ELISA Kit; Mannose-binding protein-associated serine protease 2 ELISA Kit; MASP-2) [Cleaved into: Mannan-binding lectin serine protease 2 A chain; Mannan-binding lectin serine protease 2 B chain] ELISA Kit
缩写：MASP2
Uniprot No.：O00187
种属：Homo sapiens (Human)
样本类型：serum, plasma, cell culture supernates, cerebrospinal fluid (CSF)
检测范围：23.44 pg/mL-1500 pg/mL
灵敏度：5.86 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human MASP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||
| Sample | Serum(n=4) | ||
| 1:200 | Average % | 101 | |
| Range % | 91-111 | ||
| 1:400 | Average % | 97 | |
| Range % | 91-108 | ||
| 1:800 | Average % | 99 | |
| Range % | 92-106 | ||
| 1:1600 | Average % | 95 | |
| Range % | 86-98 |
回收率：
| The recovery of human MASP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 95 | 85-99 | ||||
| EDTA plasma (n=4) | 96 | 90-112 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected 15002.5402.5392.5402.395 7502.0222.0272.0251.880 3751.1541.1901.1721.027 187.50.7020.7600.7310.586 93.750.3800.3910.3860.241 46.880.2800.3000.2900.145 23.440.1750.1610.1680.023 00.1400.1500.145 | ||||||
货期：3-5 working days
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