首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人微管相关蛋白2(MAP-2)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的MAP2含量。MAP2 即微管相关蛋白 2，在神经系统发育、维持神经细胞形态和功能等方面发挥重要作用。研究机制方面，它能促进微管的组装与稳定，调节微管动力学，参与神经突触的形成和可塑性等过程，对深入理解神经系统疾病的发病机制有重要意义。试剂盒检测范围为0.156 ng/mL-10 ng/mL，适用于神经生物学基础研究，例如体外培养神经元模型、脑组织样本或神经相关疾病动物模型中MAP-2蛋白的动态变化检测，为探索神经退行性疾病病理机制、药物干预效果评估或神经再生研究提供可靠的实验工具本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：DKFZp686I2148 ELISA Kit; MAP 2 ELISA Kit; MAP dendrite specific ELISA Kit; MAP-2 ELISA Kit; MAP2 ELISA Kit; MAP2A ELISA Kit; MAP2B ELISA Kit; MAP2C ELISA Kit; Microtubule associated protein 2 ELISA Kit; Microtubule-associated protein 2 ELISA Kit; MTAP2_HUMAN ELISA Kit
缩写：MAP2
Uniprot No.：P11137
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：0.156 ng/mL-10 ng/mL
灵敏度：0.039 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human MAP-2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 92 | ||||
| Range % | 86-95 | |||||
| 1:2 | Average % | 104 | ||||
| Range % | 100-107 | |||||
| 1:4 | Average % | 92 | ||||
| Range % | 87-95 | |||||
| 1:8 | Average % | 97 | ||||
| Range % | 91-102 | |||||
回收率：
| The recovery of human MAP-2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 93 | 89-96 | ||||
| EDTA plasma (n=4) | 97 | 90-99 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected101.6261.6131.6201.52851.1941.1761.1851.0932.50.8480.8320.8400.7481.250.6020.5980.6000.5080.6250.4280.4120.4200.3280.3120.2640.2860.2750.1830.1560.1580.1540.1560.06400.0930.0910.092 | ||||||
货期：3-5 working days
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