首页 / 产品 / ELISA试剂盒 / 大鼠ELISA试剂盒
产品描述：大鼠丙二酸单酰辅酶A(malonyl CoA)酶联免疫试剂盒为竞争法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的malonyl CoA含量。Malonyl CoA即丙二酸单酰辅酶A，是脂肪酸合成过程中的关键中间产物。其在细胞能量代谢、脂肪酸合成及氧化平衡等方面发挥重要作用。研究聚焦于其合成与代谢调控机制，如关键酶的作用，以深入了解代谢紊乱疾病发病机制并探索潜在治疗靶点。试剂盒检测范围为1.25 ng/mL-20 ng/mL，能量代谢调控机制、肥胖及糖尿病模型研究、心血管疾病相关脂代谢异常探索、脂肪酸代谢通路研究、药物干预效果评估、营养代谢调控实验本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：malonyl CoA
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma, tissue homogenates
检测范围：1.25 ng/mL-20 ng/mL
灵敏度：0.625 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Others
测定原理：quantitative
测定方法：Competitive
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat malonyl CoA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:100 | Average % | 98 | ||||
| Range % | 92-103 | |||||
| 1:200 | Average % | 102 | ||||
| Range % | 100-104 | |||||
| 1:400 | Average % | 93 | ||||
| Range % | 88-97 | |||||
| 1:800 | Average % | 89 | ||||
| Range % | 84-93 | |||||
回收率：
| The recovery of rat malonyl CoA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 95 | 90-99 | ||||
| EDTA plasma (n=4) | 84 | 80-88 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2Average 200.1020.1000.101 100.2140.2060.210 50.3850.3570.371 2.50.6720.6190.646 1.251.1211.0861.104 01.8511.7291.790 | ||||||
货期：3-5 working days
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