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产品描述：人丙二酸单酰辅酶A(malonyl CoA)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的malonyl CoA含量。Malonyl CoA是一种脂肪酸生物合成的底物，同时也是脂肪酸氧化过程中的抑制剂。它在脂肪酸合成中提供2碳单元，并抑制脂肪酸与肉碱结合，阻止其进入线粒体进行氧化。研究显示，Malonyl CoA在调节脂肪酸合成与氧化之间起着关键作用，其浓度变化对酮体生成和能量代谢有重要影响。试剂盒检测范围为0.625 ng/mL-40 ng/mL，适用于科研领域中对肝脏、脂肪组织等代谢活跃器官的功能研究，也可用于探索肥胖、糖尿病等代谢性疾病模型中malonyl CoA的动态变化及分子机制，为体外实验样本的定量分析提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：malonyl CoA
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：0.625 ng/mL-40 ng/mL
灵敏度：0.156 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Others
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human malonyl CoA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:100 | Average % | 86 | ||||
| Range % | 80-92 | |||||
| 1:200 | Average % | 96 | ||||
| Range % | 92-100 | |||||
| 1:400 | Average % | 100 | ||||
| Range % | 93-110 | |||||
| 1:800 | Average % | 91 | ||||
| Range % | 85-100 | |||||
回收率：
| The recovery of human malonyl CoA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 90 | 83-95 | ||||
| EDTA plasma (n=4) | 95 | 90-100 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mllOD1OD2AverageCorrected402.4392.2522.3462.241201.8571.7601.8091.704101.3401.3041.3221.21750.9030.8900.8970.7922.50.5150.5040.5100.4051.250.3420.3190.3310.2260.6250.2130.2030.2080.10300.1090.1010.105 | ||||||
本试剂盒所含材料：
A micro ELISA plate --- The 96-well plate has been pre-coated with an anti-human Malonyl CoA antibody. This dismountable microplate can be divided into 12 x 8 strip plates.
Two vials lyophilized standard --- Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
Biotin-labeled Malonyl CoA antibody (100 x concentrate) 1 x 120 μl ---Act as the detection antibody.
HRP-avidin (100 x concentrate) 1 x 120 μl --- Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
Biotin-antibody Diluent 1 x 15 ml ---Dilute the high concentration Biotin-antibody to an appropriate working solution.
HRP-avidin Diluent 1 x 15 ml ---Dilute the high concentration HRP-avidin solution to an appropriate solution.
本试剂盒不含材料：
A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
An incubator that can provide stable incubation conditions up to 37°C±5°C.
Centrifuge
Vortex
Squirt bottle, manifold dispenser, or automated microplate washer
Absorbent paper for blotting the microtiter plate
货期：3-5 working days
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