首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人结肠癌病灶转移相关蛋白1(MACC1)酶联免疫试剂盒双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的MACC1含量。MACC1（结肠癌转移相关基因1）是一种在多种肿瘤中表达异常升高的基因，与肿瘤转移和患者预后密切相关。其研究机制主要涉及维持肿瘤相关信号活性、转录激活促癌基因、调控上皮间质转化和促进肿瘤血管形成等方面。MACC1的表达受非编码RNA和SNP等调控，有望成为抗肿瘤治疗的新靶点。试剂盒检测范围为47 pg/mL-3000 pg/mL，适用于基础医学研究中肿瘤标志物表达分析、癌症转移相关信号通路探索、药物干预效果评估等场景，为科研人员探究MACC1在肿瘤进展中的作用机制提供标准化工具本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：7A5 ELISA Kit; MACC1 ELISA Kit; MACC1_HUMAN ELISA Kit; Metastasis associated in colon cancer 1 ELISA Kit; Metastasis-associated in colon cancer protein 1 ELISA Kit; OTTHUMP00000201891 ELISA Kit; Putative binding protein 7a5 ELISA Kit; SH3 domain-containing protein 7a5 ELISA Kit; SH3BP4L ELISA Kit
缩写：MACC1
Uniprot No.：Q6ZN28
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：47 pg/mL-3000 pg/mL
灵敏度：11.75 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cancer
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human MACC1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 96 | |||||
| Range % | 92-101 | ||||||
| 1:2 | Average % | 84 | |||||
| Range % | 80-88 | ||||||
| 1:4 | Average % | 91 | |||||
| Range % | 85-95 | ||||||
| 1:8 | Average % | 98 | |||||
| Range % | 94-102 | ||||||
回收率：
| The recovery of human MACC1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 106 | 101-113 | |||||
| EDTA plasma (n=4) | 86 | 82-90 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| pg/mlOD1OD2AverageCorrected30002.7112.5412.6262.51215001.9551.8741.9151.8017501.0441.1051.0750.9613750.5880.6120.6000.486187.50.3530.3400.3470.233940.2360.2530.2450.131470.1780.1690.1740.06000.1110.1170.114 | |||||||
货期：3-5 working days
×
