Mouse Lipoxin A4,LXA4 ELISA Kit

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

货号: IPD-E13268m

Price： ￥2500

48T 96T

数量：

大包装询价

产品描述：小鼠脂氧素A4(LXA4)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、组织匀浆样本中的LXA4含量。LXA4（脂氧素A4）是一种内源性脂氧合酶衍生的类二十烷酸调节剂，具有强大的抗炎和促消炎双重特性。其研究机制涉及抑制与ERK1/2和NF-kB通路相关的炎症反应，并在多种生理和病理过程中发挥重要作用。试剂盒检测范围为62.5 pg/mL-4000 pg/mL，适用于体外实验环境中探究炎症反应调控机制、药物干预对脂氧素通路的影响，或评估疾病模型中LXA4的代谢动态，为炎症相关疾病基础研究、药物开发前效评价等提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：LXA4
种属：Mus musculus (Mouse)
样本类型：serum, plasma, cell culture supernates, tissue homogenates
检测范围：62.5 pg/mL-4000 pg/mL
灵敏度：15.6 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Others
测定原理：quantitative
测定方法：Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse LXA4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	85
	Range %	80-90
1:2	Average %	98
	Range %	93-103
1:4	Average %	95
	Range %	90-100
1:8	Average %	94
	Range %	89-99

回收率：

The recovery of mouse LXA4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	96	90-102
EDTA plasma (n=4)	93	88-99

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/mlOD1OD2AverageCorrected40001.9571.9521.9551.84020001.5721.4761.5241.40910001.1051.1011.1030.9885000.7160.6590.6880.5732500.4480.4270.4380.3231250.3070.2990.3030.18862.50.2180.2030.2110.09600.1190.1100.115