首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人白三烯E4(LTE4)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的LT-E4含量。试剂盒检测范围为0.625 ng/mL-40 ng/mL，适用于炎症机制研究、药物筛选评估或疾病模型（如哮喘、过敏性鼻炎）中的生物标志物检测；科研人员可通过该产品探究LTE4在炎症信号通路中的作用，或评估抗炎药物对白三烯代谢的调控效果，为呼吸道疾病及免疫相关研究提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：LT-E4
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：0.625 ng/mL-40 ng/mL
灵敏度：0.156 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Biochemicals
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human LT-E4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 88 | ||||
| Range % | 83-95 | |||||
| 1:2 | Average % | 95 | ||||
| Range % | 91-98 | |||||
| 1:4 | Average % | 95 | ||||
| Range % | 88-100 | |||||
| 1:8 | Average % | 98 | ||||
| Range % | 91-103 | |||||
回收率：
| The recovery of human LT-E4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 85 | 81-93 | ||||
| EDTA plasma (n=4) | 96 | 93-105 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected 402.9752.9622.9692.819 202.5372.5742.5562.406 101.6461.7691.7081.558 50.8190.8870.8530.703 2.50.4710.4910.4810.331 1.250.2330.2510.2420.092 0.6250.2110.2010.2060.056 00.1480.1520.150 | ||||||
货期：3-5 working days
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