首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠白三烯B4(LTB4)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、组织匀浆样本中的LT-B4含量。LT-B4，即白三烯B4，是一种由花生四烯酸代谢生成的生物活性物质，具有高度炎症反应性。它在急性髓系白血病（AML）患者中表达水平异常，与不良预后相关，可能通过调控免疫微环境、促进肿瘤增殖和侵袭等机制影响AML的发生发展。试剂盒检测范围为15.6 pg/mL-1000 pg/mL，适用于体外实验中对炎症机制、免疫调节或药物干预效果的评估研究；为炎症性疾病模型研究、免疫调控机制探索等科研项目提供可靠的实验数据支持。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：N/A
缩写：LT-B4
种属：Mus musculus (Mouse)
样本类型：serum, plasma, cell culture supernates, tissue homogenates
检测范围：15.6 pg/mL-1000 pg/mL
灵敏度：3.9 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse LT-B4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 97 | ||||
| Range % | 92-104 | |||||
| 1:2 | Average % | 91 | ||||
| Range % | 86-97 | |||||
| 1:4 | Average % | 95 | ||||
| Range % | 91-99 | |||||
| 1:8 | Average % | 95 | ||||
| Range % | 89-99 | |||||
回收率：
| The recovery of mouse LT-B4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 95 | 91-101 | ||||
| EDTA plasma (n=4) | 93 | 89-97 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected10002.3342.2562.2952.1305001.8761.9341.9051.7402501.5751.5171.5461.3811251.1761.0891.1330.96862.50.6970.7330.7150.55031.20.4680.4380.4530.28815.60.2940.2870.2910.12600.1600.1690.165 | ||||||
货期：3-5 working days
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